Evaluation of Two Methods of Isolation, Differentiation and Staining of Lymphocytes by Microlymphocytotoxicity Assay

Authors

  • Glicélia da Rosa Cesumar
  • Alessandra Valéria de Oliveira Cesumar
  • Sueli Donizete Borelli UEM
  • Luciene Christina Marques da Silva Pereira UEM

Keywords:

microlinfocitotoxicidade, prova cruzada, anticorpos, microlymphocytotoxicity, cross match, antibodies

Abstract

Microlymphocytotoxicity assay is routinely used to develop the cross match. Donor lymphocytes, in this assay, are target to anti-HLA alloantibodies that could be present in the receptor sera and because of that, is important to define the methodology of isolation, differentiation and staining of lymphocytes. Due to cellular fragility of lymphocytes, mainly B lymphocytes, methodology of isolation, differentiation and staining show direct influence in the reaction. The aim of this study was to compare two methodologies of isolation, differentiation and staining of lymphocytes by ficoll-hypaque/fluorobeads and tripan blue/fluoroquent respectively. Twenty-seven reactions were developed using ficoll-hypaque/tripan and 42 by fluorobeads/fluoroquent methods. The results showed that fluorobeads/fluoroquent method had better performance in the process of isolation, differentiation and staining for both lymphocytes populations, T and B.

Author Biographies

Glicélia da Rosa, Cesumar

Acadêmica do curso de Ciências Biológicas do Centro Universitário de Maringá – CESUMAR.

Alessandra Valéria de Oliveira, Cesumar

Docente do Departamento de Ciências Biológicas do Centro Universitário de Maringá – CESUMAR.

Sueli Donizete Borelli, UEM

Docente do Departamento de Análises Clínicas da Universidade Estadual de Maringá – UEM.

Luciene Christina Marques da Silva Pereira, UEM

Acadêmica do curso de Ciências Biológicas da Universidade Estadual de Maringá – UEM.

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Published

2007-11-20

How to Cite

Rosa, G. da, Oliveira, A. V. de, Borelli, S. D., & Pereira, L. C. M. da S. (2007). Evaluation of Two Methods of Isolation, Differentiation and Staining of Lymphocytes by Microlymphocytotoxicity Assay. Iniciação Científica Cesumar, 9(2), 89–94. Retrieved from https://periodicos.unicesumar.edu.br/index.php/iccesumar/article/view/546

Issue

Vol. 9 No. 2 (2007)

Section

Artigos Originais

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