Avaliação de Dois Métodos de Separação, Diferenciação e Coloração de Linfócitos por meio da Reação de Microlinfocitotoxicidade

Glicélia da Rosa; Alessandra Valéria de Oliveira; Sueli Donizete Borelli; Luciene Christina Marques da Silva Pereira

Evaluation of Two Methods of Isolation, Differentiation and Staining of Lymphocytes by Microlymphocytotoxicity Assay

Glicélia da Rosa Cesumar
Alessandra Valéria de Oliveira Cesumar
Sueli Donizete Borelli UEM
Luciene Christina Marques da Silva Pereira UEM

Abstract

Microlymphocytotoxicity assay is routinely used to develop the cross match. Donor lymphocytes, in this assay, are target to anti-HLA alloantibodies that could be present in the receptor sera and because of that, is important to define the methodology of isolation, differentiation and staining of lymphocytes. Due to cellular fragility of lymphocytes, mainly B lymphocytes, methodology of isolation, differentiation and staining show direct influence in the reaction. The aim of this study was to compare two methodologies of isolation, differentiation and staining of lymphocytes by ficoll-hypaque/fluorobeads and tripan blue/fluoroquent respectively. Twenty-seven reactions were developed using ficoll-hypaque/tripan and 42 by fluorobeads/fluoroquent methods. The results showed that fluorobeads/fluoroquent method had better performance in the process of isolation, differentiation and staining for both lymphocytes populations, T and B.

Author Biographies

Glicélia da Rosa, Cesumar

Acadêmica do curso de Ciências Biológicas do Centro Universitário de Maringá – CESUMAR.

Alessandra Valéria de Oliveira, Cesumar

Docente do Departamento de Ciências Biológicas do Centro Universitário de Maringá – CESUMAR.

Sueli Donizete Borelli, UEM

Docente do Departamento de Análises Clínicas da Universidade Estadual de Maringá – UEM.

Luciene Christina Marques da Silva Pereira, UEM

Acadêmica do curso de Ciências Biológicas da Universidade Estadual de Maringá – UEM.

Artigo_Pdf (Português (Brasil))

Published

2007-11-20

Issue

Vol 9 No 2 (2007)

Section

Artigos Originais

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