In vitro antagonistic activity of endophytic fungi isolated from two aquatic macrophytes against phytopathogens
DOI:
https://doi.org/10.17765/2176-9168.2024v17n2e11072Keywords:
Endophytes, Aquatic plants, BiotechnologyAbstract
Endophytic microorganisms are microorganisms that live in plant tissues and occupy intercellular or intracellular spaces. They can occur in the same niche as phytopathogens, so they can be considered biological controllers that confer host protection and resistance to phytopathogens. The aim of this study was to investigate the in vitro antagonistic activity against the phytopathogens Colletotrichum sp. and Alternaria alternata. The in vitro antagonism test was statistically evaluated by analysis of variance (ANOVA) and the mean values were compared with the Scott-Knott test. The endophytic isolates with the highest antagonism indices against the phytopathogen Colletotrichum sp. were AZ13 (Alternaria palandui - KF852593) with 70.87% and AZ26 (Bipolaris papendorfii - KF852597) with 81.15%. Against the phytopathogen Alternaria alternata, endophytes AZ26 (Bipolaris papendorfii - KF852597) and AZ33 (Pestalotiopsis sp. - KF852598) had the highest antagonism indices with 49 and 57 %, respectively. These results suggest that these fungi may be promising for biocontrol of these phytopathogens.References
AGUNBIADE, Foluso. O.; OLU-OWOLABI, Bamidele. I.; ADEBOWALE, Kayode. O. Phytoremediation potential of Eichornia crassipes in metal-contaminated coastal water. Bioresource Technology, Amsterdã, v. 100, n. 19, p. 4521–4526, 1 out. 2009. https://doi.org/10.1016/j.biortech.2009.04.011
AICH, Shritama et al. Genome-wide characterization of cellulases from the hemi-biotrophic plant pathogen, Bipolaris sorokiniana, reveals the presence of a highly stable GH7 endoglucanase. Biotechnol. Biofuels, Berlim, v. 10, n. 1, p. 1-14, 2017. https://doi.org/10.1186/s13068-017-0822-0
ALMEIDA, Tiago Tognolli et al. Molecular characterization of the endophytic fungal community associated with Eichhornia azurea (Kunth) and Eichhornia crassipes (Mart.) (Pontederiaceae) native to the Upper Paraná River floodplain, Brazil. Genet. Mol. Res,. Ribeirão Preto, v. 1, n.10, p.4920-4931, 2015. https://doi.org/10.4238/2015.May.11.25
ALMEIDA, Tiago Tognolli et al. Curvulin and spirostaphylotrichins R and U from extracts produced by two endophytic Bipolaris sp. associated to aquatic macrophytes with antileishmanial activity. Nat. Prod. Res., Oxfordshire, v. 32, n. 23, p. 2783-2790, 2018. https://doi.org/10.1080/14786419.2017.1380011
ARNOLD, A. Elizabeth et al. Fungal endophytes limit pathogen damage in a tropical tree. Proc. Natl. Acad. Sci, Washington, v. 100, n. 26, p. 15649-15654, 2003. https://doi.org/10.1073/pnas.2533483100
AZEVEDO, João Lúcio et al. Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Electronic Journal of Biotechnology. v. 3, n. 1, p. 15-16, 2000.
AZEVEDO, Júlio César Rodrigues et al. Contribuição da decomposição de macrófitas aquáticas (Eichhornia azurea) na matéria orgânica dissolvida. Oecologia Brasiliensis, Rio de Janeiro, v. 12, n. 1, p. 5, 2008.
BACON, C. W. et al. Biological control of Fusarium moniliforme in maize. Environ. Health Perspect, Michigan, v. 109, n. suppl 2, p. 325-332, 2001. https://doi.org/10.1289/ehp.01109s2325
BADALYAN, Susanna M.; GARIBYAN, Narine G.; INNOCENTI, Gloria. Antagonistic activity of xylotrophic mushrooms against pathogenic fungi of cereals in dual culture.Phytopathol. Mediterr, Florença, p. 1000-1006, 2002. https://doi.org/10.14601/Phytopathol_Mediterr-1668
BERNARDI-WENZEL, Juliana et al. Molecular characterization by amplified ribosomal DNA restriction analysis and antimicrobial potential of endophytic fungi isolated from Luehea divaricata (Malvaceae) against plant pathogenic fungi and pathogenic bacteria. Genet Mol Res, Ribeirão Preto, v. 12, n. 4, p. 5072-5084, 2013. https://doi.org/10.4238/2013.october.29.2
BEGUM, Maioria. M. et al. Antagonistic potential of selected fungal and bacterial biocontrol agents against Colletotrichum truncatum of soybean seeds. Pertanika J. Trop. Agric. Sci, Putra, v. 31, n. 1, p. 45-53, 2008.
BHUNJUN, Chitrabhanu S. et al. Investigating species boundaries in Colletotrichum. Fungal Divers., Kunming , v. 107, n. 1, p. 107-127, 2021. https://doi.org/10.1007/s13225-021-00471-z
CAMPANILE, Grazia.; RUSCELLI, Angela.; LUISI, Nicola. Antagonistic activity of endophytic fungi towards Diplodia corticola assessed by in vitro and in planta tests. European Journal of Plant Pathology, Wageningen, v. 117, n. 3, p. 237-246, 2007. https://doi.org/10.1007/s10658-006-9089-1
COSTA NETO, Pedro de Queiroz. Isolamento e identificação de fungos endofíticos da pupunha (Bactris gasipaes Kunth) e caracterização por marcadores moleculares, São Carlos, 2002 https://repositorio.ufscar.br/handle/ufscar/5450
CUZZI, Claucia et al. Enzimas extracelulares produzidas por fungos endofíticos isolados de Baccharis Dracunculifolia DC (ASTERAECEAE). Global Science and Technology, v. 4, n. 2, 2011. ISSN 1984-3801
KUAN, Chee Sian et al. Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis. DNA Res.,Oxonia, v. 22, n. 3, p. 219-232, 2015. https://doi.org/10.1093/dnares/dsv007
LOU, Jingfeng et al. Metabolites from Alternaria fungi and their bioactivities. Molecules, v. 18, n. 5, p. 5891-5935, 2013. https://doi.org/10.3390/molecules18055891
MAHARACHCHIKUMBURA, Sajeewa SN et al. Pestalotiopsis revisited. Studies in Mycology, Utrecht, v. 79, n. 1, p. 121-186, 2014. https://doi.org/10.1016/j.simyco.2014.09.005
MENDES, Rodrigo.; AZEVEDO, João. Lúcio. Valor biotecnológico de fungos endofíticos isolados de plantas de interesse econômico. Micologia: avanços no conhecimento. Sociedade Brasileira de Micologia, Recife, p. 129-140, 2007.
MISHRA, Jitendra.; ARORA, Naveen. K. Secondary metabolites of fluorescent pseudomonads in biocontrol of phytopathogens for sustainable agriculture. Applied Soil Ecology, Amsterdã, v. 125, p. 35-45, 2018. https://doi.org/10.1016/j.apsoil.2017.12.004
MODENES, Aparecido N. et al. Removal of heavy metals Cd (II), Cu (II) and Zn (II) biosorption process by using the macrophyte Eicchornia crassipes. REM-REVISTA ESCOLA DE MINAS, Ouro preto v. 66, n. 3, p. 355-362, 2013. https://doi.org/10.1590/S0370-44672013000300013
OLIVEIRA, Ana Paula de. Avaliação da influência dos macronutrientes na bioacumulação do chumbo pela Eichhornia crassipes. 2012. 134 f. Dissertação (Mestrado em Engenharia Química) - Universidade Estadual do Oeste do Paraná, Toledo, 2012. https://tede.unioeste.br/handle/tede/1900
ORLANDELLI, Ravely Casarotti et al. Antifungal and proteolytic activities of endophytic fungi isolated from Piper hispidum Sw. Brazilian Journal of Microbiology, v. 46, p. 359-366, 2015. https://doi.org/10.1590/S1517-838246220131042
ORLANDELLI, Ravely Casarotti et al. Diversity of endophytic fungal community associated with Piper hispidum (Piperaceae) leaves. Genet Mol Res, Ribeirao preto v. 11, n. 2, p. 1575-1585, 2012. http://dx.doi.org/10.4238/2012.May.22.7
OWNLEY, Bonnie. H.; GWINN, Kimberly. D.; VEGA, Fernando. E. Endophytic fungal entomopathogens with activity against plant pathogens: ecology and evolution. BioControl, Berlim, v. 55, n. 1, p. 113-128, 2010. https://doi.org/10.1007/s10526-009-9241-x
POLLI, Andressa Domingos et al. Atividade antagonística dos fungos endofíticos Phoma sp. e Alternaria sp. isolados de folhas de Sapindus saponaria contra o fitopatógeno Fusarium solani. BBR-Biochemistry and Biotechnology Reports, Londrina, v. 2, n. 3 esp, p. 320-323, 2013. https://doi.org/10.5433/2316-5200.2013V2N3ESPP320
POTT, V. Joana.; POTT, Arnildo. Plantas aquáticas do Pantanal. Brasília, DF: Embrapa Comunicação para Transferência de Tecnologia; Corumbá: Embrapa-CPAP, 2000. 404 p.
SILVA, C. V.; HENRY, R. Aquatic macroinvertebrates associated with Eichhornia azurea (Swartz) Kunth and relationships with abiotic factors in marginal lentic ecosystems (São Paulo, Brazil). Brazilian Journal of Biology, São Paulo, v. 73, p. 149-162, 2013. https://doi.org/10.1590/S1519-69842013000100016
STROBEL, Gary.; DAISY, Bryan. Bioprospecting for microbial endophytes and their natural products. Microbiology and molecular biology reviews, Washington, v. 67, n. 4, p. 491-502, 2003. https://doi.org/10.1128/MMBR.67.4.491-502.2003
TOZLU, Elif. et al. Investigation on the biological control of Alternaria alternata. Indian Journal of Agricultural Sciences, v. 88, n. 8, p. 1241-1247, 2018.
WANG, Jianfeng et al. Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei. FEMS Microbiology Letters, Oxonia, v. 193, n. 2, p. 249-253, 2000. https://doi.org/10.1111/j.1574-6968.2000.tb09432.x
Downloads
Published
How to Cite
Issue
Section
License
A Revista se reserva o direito de efetuar, nos originais, alterações de ordem normativa, ortográfica e gramatical, com o intuito de manter o padrão culto da língua, respeitando, porém, o estilo dos autores. As opiniões emitidas pelos autores são de sua exclusiva responsabilidade.Os direitos autorais pertencem exclusivamente aos autores. Os direitos de licenciamento utilizado pelo periódico é a licença Creative Commons Attribution
