In vitro development of Epidendrum secundum Jacq. in simplified growth media

  • Raquel Massaro Centro Universitário Hermínio Ometto - UNIARARAS
  • Germana Marcelino Cordeiro Centro Universitário Hermínio Ometto - UNIARARAS
  • Thiago de Souza-Leal Centro Universitário Hermínio Ometto - UNIARARAS
  • Cristiano Pedroso-de-Moraes Centro Universitário Hermínio Ometto - UNIARARAS
Keywords: Orchid, In vitro propagation, Growth medium, Crop production

Abstract

The in vitro seeding is an indispensable tool to propagate the main commercial species of orchids, such as Epidendrum secundum, a species of great importance in obtaining hybrids. The present work aimed to study aspects of the in vitro development of this species by assessing the effect of MS medium with half of its concentration of macronutrients and two media based on fertilizers, Hyponex and Kristalon Laranja. The culture media were autoclaved at 121 ° C and 1 atm pressure for 20 minutes. The seeds were disinfected by being stirred in Eppendorf ® tubes, containing 5% sodium hypochlorite, for five minutes. Subsequently, they were washed and inoculated in flasks with 1 ml of distilled water. Four flasks were seeded per treatment with 1 g of seeds each. After 180 days of cultivation, the most efficient medium for the development of in vitro plantlets was inferred to be the MS medium with half of its concentration of acronutrients, which had the highest means for all the biometric variables assessed. Therefore, it can be commercially used by small producers since it presents a lower production cost.

Author Biographies

Raquel Massaro, Centro Universitário Hermínio Ometto - UNIARARAS
Discente de Iniciação Científica - Lab. de Botânica e Meio Ambiente - Centro Universitário Hermínio Ometto - UNIARARAS; E-mail: raquelmassaro@hotmail.com
Germana Marcelino Cordeiro, Centro Universitário Hermínio Ometto - UNIARARAS
Discente de Iniciação Científica - Lab. de Botânica e Meio Ambiente - Centro Universitário Hermínio Ometto - UNIARARAS; E-mail: germanacordeiro@hotmail.com
Thiago de Souza-Leal, Centro Universitário Hermínio Ometto - UNIARARAS
Discente de Iniciação Científica - Lab. de Botânica e Meio Ambiente - Centro Universitário Hermínio Ometto - UNIARARAS; E-mail: thiagosouzaleal@hotmail.com
Cristiano Pedroso-de-Moraes, Centro Universitário Hermínio Ometto - UNIARARAS
Docente e Membro do Núcleo de Ciências Ambientais Centro Universitário Hermínio Ometto - UNIARARAS; Doutorando Depto de Botânica pela Universidade Estadual Paulista - IB/UNESP; E-mail: pedroso@uniararas.br
Published
2011-05-26
Section
Environment