ANALYSIS OF TISSUE RESPONSE AND COLON ANTINEUROINFLAMMATORY ACTIVITY OF METHYL JASMONATE IN CHRONIC ULCERATIVE RECTOCOLITIS INDUCED BY TRINITROBENZENOSULFONIC ACID IN RATS

  • Alefe Luiz Caliani Carrera Centro Universitário de Maringá – UNICESUMAR
  • Isabela Ferraro Moreno Centro Universitário de Maringá – UNICESUMAR
  • Jean Carlos Fernando Besson Centro Universitário de Maringá – UNICESUMAR
  • Maria Raquel Marçal Natali Universidade Estadual de Maringá – UEM
DOI: https://doi.org/10.17765/1518-1243.2019v21n2p153-162
Keywords: Intestinal inflammatory diseases, Jasminum, Intestinal mucus, Regeneration, Enteric nervous system

Abstract

Intestine inflammatory diseases (IIDs) are characterized by a chronic inflammatory processes mainly, Crohn Disease (CD) and Ulcerative Rectocolites (URC). Natural products suggest therapeutic alternatives such as methyl jasmonate (MeJA) as a probable alternative treatment, with proven anti-inflammatory, anti-proliferative and antioxidant activities. The compound is isolated from the essential oil of Jasminum grandiflorum. Potential tissue and neuro-inflammatory effect of MeJA at 300 mg/kg in adult rats with colitis was induced by trinitrobenzenosulfonic acid (TNBS). Rats were divided into four groups and euthanized after treatment. Samples of distal colon of 20 animals were employed to obtain transversal histological cuts. Morphometric analyses of intestine tunics were undertaken by samples of images by optic microscope; antineuroinflammatory profile of the enteric nervous system was also undertaken by microscopic evaluation of 30 ganglia in the myoenteric ganglia per animal in counting and characterization of inflammatory cells. Efficient simulation of IID by TNBS was reported by reduction of inflammatory cells in enteric ganglia, especially lymphocytes and monocytes; tissue regenerative effect in the submucus and muscular tunics.

Author Biographies

Alefe Luiz Caliani Carrera, Centro Universitário de Maringá – UNICESUMAR
Discente do curso de Medicina Veterinária, Centro Universitário de Maringá – UNICESUMAR, Maringá, PR, Brasil.. Bolsista PIC/ICETI UniCesumar
Isabela Ferraro Moreno, Centro Universitário de Maringá – UNICESUMAR
Discente do curso de Medicina Veterinária, Centro Universitário de Maringá – UNICESUMAR
Jean Carlos Fernando Besson, Centro Universitário de Maringá – UNICESUMAR
Orientador. Doutor em ciências biológicas (UEM). Docente do curso de Medicina, Centro Universitário de Maringá – UNICESUMAR, Maringá, PR, Brasil
Maria Raquel Marçal Natali, Universidade Estadual de Maringá – UEM
Doutora em ciências biológicas (UNESP). Docente do departamento de Ciências Morfológicas, Universidade Estadual de Maringá – UEM, Maringá, PR

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Published
2019-12-13
Issue
Vol 21 No 2 (2019): Temática: Medicina Veterinária e Zootecnia
Section
Artigos de Iniciação Científica
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