Effects of pre-slaughter stress on the quality of tambaqui meat stored on ice
DOI:
https://doi.org/10.17765/2176-9168.2023v16n3e10717Keywords:
Amazon, animal production, Colossoma macropomum, stress, Fish meat qualityAbstract
The objective of this study was to evaluate the effects of stress caused by different pre-slaughter protocols on physicochemical, sensory and microbiological quality of tambaqui meat stored on ice for 49 days. A total of 144 Tambaqui fish were used. The experimental design was completely randomized constituted by six treatments related to the pre-slaughter protocols (slaughter right after harvesting, after 3h transport, and after 6h, 12h, 24h or 44h recovery period after transport). After confirming the death, fish were placed in styrofoam boxes with ice. Every seven days, three fish from each treatment were collected from the styrofoam boxes for physical, chemical, sensory and microbiological analyzes. Data collected were subjected to ANOVA and, subsequently, to the Tukey test at 0.01 and 0.05. Fish slaughtered under stress right after harvesting and transport presented fillets with higher bacterial growth rates, and worst values of pH, NBVT and TBARS, in addition to producing fillets with a darker color than other treatments in the first 21 days. These samples were considered unsuitable for consumption after 42 days stored on ice. Fish submitted to recovery periods after transport presented better meat quality, where fillets presented more luminous and less red color after 21 days stored on ice, as well as lower microbiological growth rates, and better values of pH, NBVT and TBARS. Our results proved that the use of recovery periods after transport and before the slaughter improves tambaqui meat quality and increases its shelf life when stored on ice up to 49 days.References
AL-KAHTANI, H.A.; ABU-TARBOUSH, H.M.; BAJABER, A.S.; ATIA, M.; ABOU-ARAB, A.A.; EL-MOJADDIDI, M.A. Chemical changes after irradiation and post-irradiation in tilapia and Spanish mackerel. J. Food Sci., v. 61, n. 4, p. 729-733, 1996. DOI: https://doi.org/10.1111/j.1365-2621.1996.tb12191.x
AMARAL, A. B.; SILVA, M. V.; LANNES, S.C.S. Lipid oxidation in meat: mechanisms and protective factors – a review. Food Sci. Technol., v. 38, p. 1-15, 2018. DOI: https://doi.org/10.1590/fst.32518
BARTON, B.A. Stress in fishes: A diversity of responses with particular reference to changes in circulating corticosteroids. Integr. Comp. Biol., v. 42, p. 517-525, 2002. DOI: https://doi.org/10.1093/icb/42.3.517
BRASIL. Regulamento da Inspeção Industrial e Sanitária de Produtos de Origem Animal – RIISPOA: pescados e derivados. Brasília: Ministério da Agricultura, Pecuária E Abastecimento / Departamento Nacional de Inspeção de Produtos de Origem Animal, 2001
CONCOLLATO, A.; PARISI, G.; OLSEN, R.E.; KVAMME, B.O.; SLINDE, E.; ZOTTE, A.D. Effect of carbon monoxide for Atlantic salmon (Salmo salar L.) slaughtering on stress response and fillet shelf life. Aquaculture, v. 14, p. 271-273, 2014. DOI: https://doi.org/10.1016/j.aquaculture.2014.05.040
DIGRE, H.; JES HANSEN, U.; ERIKSON, U. Effect of trawling with traditional and ‘T90’ trawl codends on fish size and on different quality parameters of cod Gadus morhua and haddock Melanogrammus aeglefinus. Fish. Sci., v. 76, p. 549-559, 2010. DOI: https://doi.org/10.1007/s12562-010-0254-2
DIGRE, H.; ERIKSON, U.; SKARET, J.; LEA, P.; GALLART JORNET, L.; MISIMI, E. Biochemical, physical and sensory quality of ice-stored Atlantic cod (Gadus morhua) as affected by pre-slaughter stress, percussion stunning and AQUI-STM anaesthesia. Eur. Food Res. Technol., v. 233, p. 447-456, 2011. DOI: https://doi.org/10.1007/s00217-011-1531-8
DIGRE, H.; ROSTEN, C.; MATHIASSEN, J.R.; AURSAND., I.G. The on-board live storage of Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) caught by trawl: Fish behaviour, stress and fillet quality. Fish. Res., v. 189, p. 42-54, 2017. DOI: https://doi.org/10.1016/j.fishres.2017.01.004
DINIZ, N.M.; HONORATO, C.A. Algumas alternativas para diminuir os efeitos do estresse em peixes de cultivo – Revisão. Arq. Ciênc. Vet. Zool. UNIPAR., v. 15, n. 2, p. 149-154, 2012.
ERIKSON, U.; MISIMI, E. Atlantic salmon skin and fillet color changes as effected by perimortem handling stress, rigor mortis, and ice storage. J. Food Sci., v. 73, p. 50-59, 2008. DOI: https://doi.org/10.1111/j.1750-3841.2007.00617.x
ERIKSON, U.; DIGRE, H.; GRIMSMO, L. Electrical immobilisation of saithe (Pollachius virens): Effects of pre-stunning stress, applied voltage, and stunner configuration. Fish. Res., v. 179, p. 148-155, 2016. DOI: http://dx.doi.org/10.1016/j.fishres.2016.02.017
GATICA, M.C.; MONTI, G.E.; KNOWLES, T.G.; GALLO, C.B. Effects of crowding on blood constituents and flesh quality variables in Atlantic salmon (Salmo salar). Arch. Med. Vet., v. 42, p. 187-193, 2010. DOI: http://dx.doi.org/10.4067/S0301-732X2010000300010
GOES, E.S.R.; LARA, J.A.F.; GASPARINO, E.; DEL VESCO, A.P.; GOES, M.D.; ALEXANDRE FILHO, L.; RIBEIRO, R.P. Pre-slaughter stress affects ryanodine receptor protein gene expression andthe water-holding capacity in fillets of the Nile tilapia. Plos One, v. 10, p. e0129145, 2015. DOI: https://doi.org/10.1371/journal.pone.0129145
GOES, E.S.R.; LARA, J.A.F.; GASPARINO, E.; GOES, M.D.; ZUANAZZI, J.S.G.; LOPERA-BARRERO, N.M.; RODRIGUEZ, M.D.P.R.; CASTRO, P.L.; RIBEIRO, R.P. Effects of transportation stress on quality and sensory profiles of Nile tilapia fillets. Sci. Agric., v. 75, p. 321-328, 2018. DOI: https://doi.org/10.1590/1678-992X-2016-0387
GOES, E.S.R.; GOES, M.D.; CASTRO, P.L.; LARA, J.A.F.; VITAL, A.C.P.; RIBEIRO, R.P. Imbalance of the redox system and quality of tilapia fillets subjected to pre-slaughter stress. PLoS ONE, v. 14, n. 1, p. e0210742, 2019. DOI: https://dx.doi.org/10.1371%2Fjournal.pone.0210742
HULTMANN, L.; PHU, T.M.; TOBIASSEN, T.; AAS-HANSEN, Ø.; RUSTAD, T. Effects of pre-slaughter stress on proteolytic enzyme activities and muscle quality of farmed Atlantic cod (Gadus morhua). Food Chem., v. 134, p. 1399-1408, 2012. DOI: https://doi.org/10.1016/j.foodchem.2012.03.038
INOUE, L.A.K.A.; HACKBARTH, A.; MORAES, G. Benzocaína sobre respostas ao estresse do matrinxã submetido ao transporte em sacos plásticos. Rev. Bras. de Saude e Prod. Anim., v.11, n.3, p.909-918, 2010.
INSTITUTO ADOLFO LUTZ. Normas Analíticas do Instituto Adolfo Lutz – Métodos químicos e físicos para análise de alimentos. 4. ed., 1 edição digital. São Paulo: Instituto Adolfo Lutz, 2008.
LARSEN, E.P.; HELDBO, J.; JESPERSEN, C.M.; NIELSEN, J. Development of a standard for quality assessment on fish consumption. In: HUSS, H.H.; JACOBSEN, M.; LISTON, J. (eds.). Quality assurance in the fish industry. Amsterdam: Elsevier, pp. 351-358, 1992.
LANZARIN, M.; RITTER, D.O.; NOVAES, S.F.; MONTEIRO, M.L.G.; ALMEIDA-FILHO, E.S.; MARSICO, E.T.; FRANCO, R.M.; CONTE-JUNIOR, C.A.; FREITAS, M.Q. Quality Index Method (QIM) for ice stored gutted Amazonian Pintado (Pseudoplatystoma fasciatum x Leiarius marmoratus) and estimation of shelf life. LWT - Food Sci. Technol., v. 65, p. 363-370, 2016. DOI: http://dx.doi.org/10.1016/j.lwt.2015.08.019
LEFEVRE, F.; COS, I.; POTTINGER, T.G.; BUGEON, J. Selection for stress responsiveness and slaughter stress affect flesh quality in pan-size rainbow trout, Oncorhynchus mykiss. Aquaculture, v. 464, p. 654-664, 2016. DOI: http://dx.doi.org/10.1016/j.aquaculture.2016.07.039
LERFALL, J.; ROTH, B.; SKARE, E.F.; HENRIKSEN, A.; BETTEN, T.; DZIATKOWIAK-STEFANIAK, M.A.; ROTABAKK, B.J. Pre-mortem stress and the subsequent effect on flesh quality of pre-rigor filleted Atlantic salmon (Salmo salar L.) during ice storage. Food Chem., v. 175, p. 157–165, 2015. DOI: 10.1016/j.foodchem.2014.11.111
MARTINS, C.V.B.; VAZ, S.K.; MINOZZO, M.G. Aspectos sanitários de pescados comercializados em pesque-pague de Toledo – PR. Hig. Aliment., v. 16, n. 98, p. 51-56, 2002.
MATOS, E.; GONÇALVES, A.; NUNES, M.L.; DINIS, M.T.; DIAS, J. Effect of harvesting stress and slaughter conditions on selected flesh quality criteria of gilthead sea bream (Sparus aurata). Aquaculture, v. 305, p. 66-72, 2010. DOI: https://doi.org/10.1016/j.aquaculture.2010.04.020
MELO, F.V.S.T.; VIEGAS, E.M.M.; PARISI, G.; BORDIGNON, A.C.; NETO, M.A.C.; MELO, J.F.B. Physical, chemical and sensory evaluation of meat from cobia (Rachycentron canadum), desensitized with different voltages of electric shock, stored under refrigeration. Ciênc. Rural, v. 49, n. 2, e20180242, 2018. DOI: https://doi.org/10.1590/0103-8478cr20180242
MENDES, J.M.; INOUE, L.A.K.A.; JESUS, R.S. Influência do estresse causado pelo transporte e método de abate sobre o rigor mortis do tambaqui (Colossoma macropomum). Braz. J. Food Technol., v. 18, n. 2, p. 162-169, 2015. DOI: http://dx.doi.org/10.1590/1981-6723.1115
MENDES, J.M.; DAIRIKI, J.K.; INOUE, L.A.K.A.; JESUS, R.S. Advantages of recovery from pre-slaughter stress in tambaqui Colossoma macropomum (Cuvier 1816) agroindustry in the Amazon. Food Sci. Technol., v. 37, p. 383-388, 2017. DOI: https://doi.org/10.1590/1678-457X.14316
NATHANAILIDES, C.; PANOPOULOS, S.; KAKALI, F.; KARIPOGLOU, C.; LENAS. D. Antemortem and postmortem biochemistry, drip loss and lipid oxidation of European sea bass muscle tissue. Procedia Food Sci., v. 1, p. 1099-1104, 2011. DOI: https://doi.org/10.1016/j.profoo.2011.09.164
OBA, E.T.; MARIANO, W.S.; SANTOS, L.R.B. Estresse em peixes cultivados: agravantes e atenuantes para o manejo rentável. Macapá: EMBRAPA, p. 226-246, 2009.
OGAWA, M. S., OGAWA, N. B. P. Alterações do pescado post-mortem. In Ogawa, M Maia, E. L. (editores). Manual de Pesca. Fortaleza: Varela, p.113-137,1999.
OLIVEIRA, P.R.; JESUS, R.S.; BATISTA, G.M.; LESSI, E. Avaliação sensorial, físico-química e microbiológica do pirarucu (Arapaima gigas) durante estocagem em gelo. Braz. J. Food Technol., v. 17, n. 1, p. 67-74, 2014. DOI: https://doi.org/10.1590/bjft.2014.010
OLIVEIRA FILHO, P.R.C.; OLIVEIRA, P.J.M.G.; MELO, M.P.; VIEGAS, E.M.M.; NATORI, M.M.; BALDI, S.C.V. Physiological stress and meat quality of pacu (Piaractus mesopotamicus) submitted to CO2 narcosis, hypothermia and electrical stunning. Aquac. Res., v. 52, p. 5034-5043, 2021. DOI: https://doi.org/10.1111/are.15375
RAHMANIFARAH, K.; SHABANPOUR, B.; SATTARI, A. Effects of clove oil on behavior and flesh quality of common carp (Cyprinus carpio) in comparison with pre-slaughter CO2 stunning, chilling and asphyxia. Turk. J. Fish. Aquat. Sci., v. 11, p. 139-147, 2011. DOI: http://dx.doi.org/10.4194/trjfas.2011.0118
SANTOS, G.M.; FERREIRA, E.J.G.; ZUANON, J.A.S. Peixes Comerciais de Manaus. 2ª ed. Manaus: Instituto Nacional de Pesquisas da Amazônia, p.144, 2009.
SCHERER, R.; DANIEL, A.P.; AUGUSTI, P.R.; LAZZARI, R.; LIMA, R. L.; FRIES, L. L.M.; RADUNZ-NETO, J.; EMANUELLI, T. Efeito do gelo clorado sobre parâmetros químicos e microbiológicos da carne de carpa capim (Ctenopharyngodon idella). Food Sci. Technol., v. 24, n. 4, p. 680-684, 2004. DOI: https://doi.org/10.1590/S0101-20612004000400034
SILVA, N.; JUNQUEIRA, V.C.A.; SILVEIRA, N.F.A. Manual de Métodos de Análise Microbiológica de Alimentos. São Paulo: Livraria Varela, 2001.
SILVA, M.L.B.P.; LOPES. J.M.; VIEIRA, F.G.A.; ARAUJO, T.D.S.; CALVET, R.M.; PEREIRA, A.M.L. Development of a quality index scheme and shelf life study for whole tambaqui (Colossoma macropomum. Acta Amaz., v. 48, n. 2, p. 98-108, 2018. DOI: https://doi.org/10.1590/1809-4392201703441
VARGAS, S.C.: OLIVEIRA FILHO, P.R.C.; NATORI, M.M.; LIMA, C.G.; VIEGAS, E.M.M. Evaluation of different stunning methods on aspects of animal welfare and meat quality of matrinxã (Brycon cephalus). Ital. J. Food Sci., v. 25, p. 255-263, 2013.
VARGAS-BALDI, S.C.V.; PARISI, G.; BONELLI, A.; BALIEIRO, J.C.C.; GUIMARÃES, J.L.; VIEGAS, M.M. Effects of different stunning/slaughter methods on frozen fillets quality of cobia (Rachycentron canadum). Aquaculture, v. 486, p. 107-113, 2018. DOI: http://dx.doi.org/10.1016/j.aquaculture.2017.12.003
VIEGAS, E.M.M.; OLIVEIRA FILHO, P.R.C.; MELO, M. P.; GIRAO, P.J.M. Indicators of stress in tilápia subjected to different stunning methods. Bol. Inst. Pesca, v. 41, p. 335-343, 2015.
VYNCKE, W. Direct determination of the thiobarbituric 34. acid value in trichloroacetic extracts of fish as a measure of oxidative rancidity. Fette-
Seifen Anstrichmittel, v. 72, n. 12, p. 1084 -1087, 1970.
WOOTLON, M.; CHUAH, S.H. The use of sea mullet (Mugil cephalus) in the production of cold marinades. Food Technology in Australia, v. 33, p. 392-397, 1981.
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