Effect of calorie restriction on thoracic aorta histomorphometry of aged rats
Abstract
The purpose of this study was to assess the effects of the calorie restriction on thoracic aorta morphology in aged rats, and, from this analysis, infer the capacity of this intervention to reduce measurable histological changes in the course of time. It was used 41 white male Wistar rats that were separated into 7 groups: control (Co) 7, 12, 18 and 23 months and calorie restriction (Rc) 12, 18 and 23 months. The rats were sacrificed and their aortas were collected and fixed in 10% formalin. The aortas were prepared through the histological routine techniques, with inclusion in paraffin followed by cuts of 6 μm, which were stained with orcein-picrosirius-hematoxylin and Masson’s trichrome. The statistic was made through analysis of variance (ANOVA) and the Tukey´s test were employed at 5% significance. The results shown lower body weight and lower intima-media thickness in the calorie restriction group compared to control groups. The effectiveness of calorie restriction in reducing the aging changes only was observed up to 18 months, with no significant difference between the control and calorie restriction groups. Therefore, it is concluded that there is an age limit for the possible attenuating effects of the calorie restriction on changes of senescence in the arterial wall.References
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