Control of Nematodes in Tomato Plants with Mutant Isolates of Paecilomyces lilacinus

  • Diogo Robl Universidade Federal do Paraná
  • Arlei Maceda Centro de Diagnóstico “Marcos Enrietti” (CDME), da Secretaria da Agricultura e do Abastecimento do Estado do Paraná.
  • Patricia do Rocio Dalzoto Universidade Federal do Paraná
  • Camila da Costa Senkiv Universidade Federal do Paraná
  • Ida Chapaval Pimentel Universidade Federal do Paraná
  • Maria Aparecida Cassilha Zawadneak Universidade Federal do Paraná
Keywords: Lycopersicon esculentum, Meloidogyne incognita, Meloidogyne javanica, biological control

Abstract

The effect of three different isolates of Paecilomyces lilacinus in the control of Meloidogyne incognita 3 and Meloidogyne javanica was evaluated. Experiment was performed in a greenhouse, between November 2008 and May 2009, with a randomized design comprising 10 treatments and 3 repetitions. P. lilacinus isolates were PAE 69 (endophyte isolade), 2K and RG3 (mutant isolates). Inoculum was produced in sterilized cooked rice and the production of nematode eggs was undertaken in tomato plants, Santa Clara cultivar, during 60 days. They were inoculated with 4000 eggs/nematode juveniles and 50 g colonized rice (109 spores. g-1 rice). After 45 days, number of galls and egg mass per root system were evaluated. Results showed that no decrease in plant parasitism by nematodes occurred with the three P. lilacinus isolates when compared to control (p-rate>0.05). Since M. incognita produces significantly (p-rate = 0.0003) a larger egg mass per gall than M. javanica, a greater reproduction capacity of the former species has been shown. Within the context of current study, the fungus isolates analyzed were not efficient for the biological control of M. incognita 3 and M. javanica.

Author Biographies

Diogo Robl, Universidade Federal do Paraná
Engenheiro de Bioprocessos e Biotecnologista na Universidade Federal do Paraná – UFPR, Ex-Bolsista de Iniciação Cientifica do Departamento de Patologia Básica, UFPR. E-mail: diogo_robl@hotmail.com
Arlei Maceda, Centro de Diagnóstico “Marcos Enrietti” (CDME), da Secretaria da Agricultura e do Abastecimento do Estado do Paraná.
Engenheiro Agrônomo, Mestre e Pesquisador do Centro de Diagnóstico “Marcos Enrietti” (CDME), da Secretaria da Agricultura e do Abastecimento do Estado do Paraná. E-mail: arleimaceda@seab.pr.gov.br
Patricia do Rocio Dalzoto, Universidade Federal do Paraná
Bióloga, Doutora e Docente do Departamento de Patologia Básica, Universidade Federal do Paraná – UFPR. E-mail: pdalzoto@ufpr.br
Camila da Costa Senkiv, Universidade Federal do Paraná
Discente do Curso de Ciências Biológicas – Universidade Federal do Paraná – UFPR, Ex-Bolsista de Iniciação Cientifica do Departamento de Patologia Básica da Universidade Federal do Paraná – UFPR. E-mail: camilacsgt@hotmail.com
Ida Chapaval Pimentel, Universidade Federal do Paraná
Engenheira Agrônoma, Doutora e Docente do Departamento de Patologia Básica – Universidade Federal do Paraná - UFPR; E-mail: ida@ufpr.br
Maria Aparecida Cassilha Zawadneak, Universidade Federal do Paraná
Engenheira Agrônoma, Doutora, Orientadora e Docente do Departamento de Patologia Básica na Universidade Federal do Paraná – UFPR. E-mail: mazawa@ufpr.br
Published
2012-05-29
Issue
Vol 14 No 2 (2012): Temática: Educação
Section
Artigos de Iniciação Científica
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