STANDARDIZATION OF EGG ECLOSION TEST: IN VITRO DIAGNOSIS OF RESISTANCE TO ALBENDAZOLE IN GASTROINTESTINAL NEMATODES
DOI:
https://doi.org/10.17765/1518-1243.2019v21n2p125-130Keywords:
Benzimidazol, Helminths, RuminantsAbstract
Current analysis standardized, adapted and detailed egg eclosion test (EET) to diagnose the resistance of gastrointestinal nematodes to albendazole in sheep. Three in vitro assays were undertaken at 24 and 48 h, by methodology prescribed by the EMBRAPA Practical Handbook, with negative control distilled water and DMSO at 0.75% and concentration at 600, 300, 150, 112.5, 75, 37.5, 18.75, 9.37 and 4.68 ?g/mL and positive control albendazole 25.000 ?g/mL. Plates were incubated in buffer with BOD and maintained at 28ºC. After 24h and 48h of incubation, larvae had eclosion in negative control groups and also in groups treated with 112.5 ?g/mL and 75 ?g/mL, respectively. It was not possible to diagnose anti-helminth resistance in EET due to limiting variables, such as the lack of discriminating dose for albendazole. The standardization, adaptation and detailing were possible to diagnose anti-helminth resistance of nematodes to albendazole.References
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