COLORED LED REDUCES ENERGY USE, AFFECTING LETTUCE SEED GERMINATION, GROWTH, AND ANTIOXIDANT ACTIVITY POSITIVELY

Ana Luiza Reale; Heloisa Santos; Giovani Tirelli; Leticia Moreno; Wilson Pereira; Elisa Bicalho

doi:10.17765/2176-9168.2024v17n.Especial.e12444

Autores

Ana Luiza Reale Universidade Federal de Lavras - UFLA https://orcid.org/0000-0002-6699-1981
Heloisa Santos Universidade Federal de Lavras - UFLA https://orcid.org/0000-0003-1384-4969
Giovani Tirelli Universidade Federal de Lavras - UFLA https://orcid.org/0000-0003-1560-9700
Leticia Moreno Universidade Federal de Lavras - UFLA https://orcid.org/0000-0002-1440-0756
Wilson Pereira Universidade Federal de Lavras - UFLA https://orcid.org/0000-0002-7931-8382
Elisa Bicalho Universidade Federal de Lavras - UFLA https://orcid.org/0000-0003-1746-0663

DOI:

https://doi.org/10.17765/2176-9168.2024v17n.Especial.e12444

Palavras-chave:

Antioxidant system; Lactuca sativa; Light emitting diode; Photooxidation; Seed quality.

Resumo

As vegetables have been growing and space has gathered on the market, there is increasing demand for alternative light sources. This research aimed to evaluate the effects of colored LEDs on the germination and initial growth of lettuce plants, as well as their effects on the antioxidant system. Seeds were germinated in a chamber at 20°C under a 12-hour photoperiod. The light treatment in the first phase consisted of white and colored light-emitting diode (LED) lights (red-V + blue-A) in proportions of 100% V, 80% V + 20% A, 50% V + 50% A, and 80% A + 20% V. The first phase of the experiment consisted of a completely randomized design in a 2x4 factorial scheme (two light conditions and four seed lots) with four replications. The first count, germination, germination speed index (GSI), root length, shoot length, total seedling length, and shoot-to-root ratio were evaluated via image analysis. For the second phase of the experiment, the quantification of antioxidative enzyme activity (SOD, CAT, and APX) was performed to assess whether the light treatments (white LED light, colored LED light, and fluorescent light) caused photooxidative damage in the seedlings. Compared with white LED light, colored LED light improved plant germination and growth by promoting faster radicle protrusion, a greater GSI, a longer total seedling length, and a longer primary root length. The quantification of SOD, CAT, and APX activity indicated that the quality of light used in this work did not cause photooxidative stress in lettuce plants.

Biografia do Autor

Ana Luiza Reale, Universidade Federal de Lavras - UFLA

Mestra em Agronomia (Fitotecnia) pela Universidade Federal de Lavras (UFLA).

Heloisa Santos, Universidade Federal de Lavras - UFLA

Doutora em Agronomia (Fitotecnia) pela Universidade Federal de Lavras (UFLA). Docente adjunta da Universidade Federal de Lavras (UFLA), Lavras, MG, Brasil.

Giovani Tirelli, Universidade Federal de Lavras - UFLA

Mestrando em Agronomia pela Universidade Federal de Lavras (UFLA).

Leticia Moreno, Universidade Federal de Lavras - UFLA

Doutora em Agronomia pela Universidade da Georgia - Campus Tifton, GA, EUA.

Wilson Pereira, Universidade Federal de Lavras - UFLA

Doutor em Agronomia (Fitotecnia) pela Universidade Federal de Lavras (UFLA).

Elisa Bicalho, Universidade Federal de Lavras - UFLA

Doutora em Biologia Vegetal pela Universidade Federal de Minas Gerais (UFMG). Docente permanente do Programa de Pós-Graduação em Fisiologia Vegetal da UFLA, Lavras, MG, Brasil.

Referências

AN, Z. F.; ZHOU, C. J. Light induces lettuce seed germination through promoting nitric oxide production and phospholipase D-derived phosphatidic acid formation. South African Journal of Botany, v. 108, p. 416-422. 2017. https://doi.org/10.1016/j.sajb.2016.09.010

ALSCHER, R. G. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany, v. 53, p. 1331–1341. 2002.https://doi.org/10.1093/jexbot/53.372.1331

BIAN, Z. et al. Effect of green light on nitrate reduction and edible quality of hydroponically grown lettuce (Lactuca sativa L.) under short-term continuous light from red and blue light-emitting diodes. Environmental and Experimental Botany, v. 153, p. 63–71. 2018. https://doi.org/10.1016/j.envexpbot.2018.05.010

BIEMELT, S.; KEETMAN, U.; ALBRECHT, G. Re-Aeration following hypoxia or anoxia leads to activation of the antioxidative defense system in roots of wheat seedlings. Plant Physiology, v. 116, p. 651–658. 1998. https://doi.org/10.1104/pp.116.2.651

BRASIL. Regras para análise de sementes. Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. Brasília: Mapa/ACS, 2009. 399 p.

CAKMAK, I.; KIRKBY, E. A. Role of magnesium in carbon partitioning and alleviating photooxidative damage. Physiology Plant, v. 133, p. 692–704. 2008. https://doi.org/10.1111/j.1399-3054.2007.01042.x

CHEN, X. L. et al. Growth and nutritional properties of lettuce affected by different alternating intervals of red and blue LED irradiation. e, v. 223, p. 44-52. 2017. https://doi.org/10.1016/j.scienta.2017.04.037

DOWDLE, J. et al. Two genes in Arabidopsis thaliana encoding GDP?L?galactose phosphorylase are required for ascorbate biosynthesis and seedling viability. The Plant Journal., v. 52, p. 673-689. 2007. https://doi.org/10.1111/j.1365-313X.2007.03266.x

FERREIRA, D. F. A computer analysis system to fixed effects split plot type designs. Revista Brasileira de Biometria, v. 37, p. 529–535. 2019. https://doi.org/10.28951/rbb.v37i4.450

FINCH-SAVAGE, W. E.; LEUBNER-METZGER, G. Seed dormancy and the control of germination. New Phytology., v. 171, p. 501–523. 2006. https://doi.org/10.1111/j.1469-8137.2006.01787.x

FRANZIN, S. M.; MENEZES, N. L.; GARCIA, D. C.; WRASSE, C. F. Methods for evaluating the physiological potential of lettuce seeds (In Portuguese). Brasilian Seed Journal, v. 26, p. 63–69. 2004. https://doi.org/10.1590/s0101-31222004000200009

FUKUNAGA, K.; FUJIKAWA, Y.; ESAKA, M. Light regulation of ascorbic acid biosynthesis in rice via light responsive cis-elements in genes encoding ascorbic acid biosynthetic enzymes. Bioscience, Biotechnology, and Biochemistry, v. 74, p. 888-891. 2010. https://doi.org/10.1271/bbb.90929

GIANNOPOLITS, C. N.; RIES, S. K. Superoxide Dismutases: II. Purification and Quantitative Relationship with Water-soluble Protein in Seedlings. Plant Physiology, v. 59, p. 315–318. 1977. https://doi.org/10.1104/pp.59.2.315

GIL, C. S.; JUNG, H. Y.; LEE, C.; EOM, S. H. Blue light and NAA treatment significantly improve rooting on single leaf-bud cutting of Chrysanthemum via upregulated rooting-related genes. Science Horticulture, v. 274, p. 109650. 2020. https://doi.org/10.1016/j.scienta.2020.109650

HAVIR, E. A.; MCHALE, N. A. Purification and characterization of an isozyme of catalase with enhanced-peroxidatic activity from leaves of Nicotiana sylvestris. Archives of Biochemistry and Biophysics, v. 283, p. 491–495. 1990. https://doi.org/10.1016/0003-9861(90)90672-L

HEYNEKE, E. et al. Dynamic compartment specific changes in glutathione and ascorbate levels in Arabidopsis plants exposed to different light intensities. BMC Plant Biology, v. 13. 2013. https://doi.org/10.1186/1471-2229-13-104

LIAO, P. A.; LIU, J. Y.; SUN, L. C.; CHANG, H. H. Can the adoption of protected cultivation facilities affect farm sustainability? Sustain, 12:1–17. 2020. https://doi.org/10.3390/su12239970

LIN, C. Blue light receptors and signal transduction. Plant Cell, v. 14, p. s207-S225. 2002. https://doi.org/10.1105/tpc.000646

LIN, K. H. et al. The effects of red, blue, and white light-emitting diodes on the growth, development, and edible quality of hydroponically grown lettuce (Lactuca sativa L. var. capitata). Science Horticulture, v. 150, p. 86–91. 2013. https://doi.org/10.1016/j.scienta.2012.10.002

LOVEGROVE, A.; HOOLEY, R. Gibberellin and abscisic acid signaling in aleurone. Trends Plant Science, v. 5, p. 102-110. 2000.

MAGUIRE, J. D. Speed of germination—Aid in selection and evaluation for seedling emergence and vigor. Crop Science, v. 2, p. 176–177. 1962. https://doi.org/10.2135/cropsci1962.0011183x000200020033x

METIVIER, J.; VIANA, A. M. The effect of long and short-day length upon the growth of whole plants and the level of soluble proteins, sugars, and stevioside in leaves of Stevia rebaudiana Bert. Journal of Experimental Botany, v. 30, p. 1211-1222. 1979

MORROW, R. C. LED lighting in horticulture. HortScience, v. 43, p. 1947–1950. 2008. https://doi.org/10.21273/hortsci.43.7.1947

MURATA, N.; TAKAHASHI, S.; NISHIYAMA, Y.; ALLAKHVERDIEV, S. I. Photoinhibition of photosystem II under environmental stress. Biochimica et Biophysica Acta, v. 1767, p. 414–421. 2007. https://doi.org/10.1016/j.bbabio.2006.11.019

NAKANO, Y.; ASADA, K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology, v. 22, p. 867–880. 1981. https://doi.org/10.1093/oxfordjournals.pcp.a076232

NOCTOR, G.; VELJOVIC-JOVANOVIC, S.; FOYER, C. H.; GRACE, S. Peroxide processing in photosynthesis: Antioxidant coupling and redox signaling. The Royal Society, v. 355, p. 1465-1475. 2000. https://doi.org/10.1098/rstb.2000.0707

OHASHI-KANEKO, K. et al. Effect of light quality on growth and vegetable quality in leaf lettuce, spinach and komatsuna. Environmental Control in Biology, v. 45, p. 189–198. 2007. https://doi.org/10.2525/ecb.45.189

OLSZEWSKI, N.; SUN, T. P.; GUBLER, F. Gibberellin signaling: Biosynthesis, catabolism, and response pathways. Plant Cell, v. 14, p. 61-80. 2002. https://doi.org/10.1105/tpc.010476

PANIAGUA-PARDO, G. et al. Efecto de la luz led de alta intensidad sobre la germinación y el crecimiento de plántulas de brócoli (Brassica oleracea L.). Polibotánica, v. 40, p. 199-212. 2015.

PENG, J. et al. The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses. Genes & Development, v. 11, p. 3194-3205. 1997.

SABIR, N.; SINGH, B. Protected cultivation of vegetables in global arena: A review. Indian Journal of Agricultural Sciences, v. 83, p. 123–135. 2013.

SABZALIAN, M. R. et al. High performance of vegetables, flowers, and medicinal plants in a red?blue LED incubator for indoor plant production. Agronomy for Sustainable Development, v. 34, p. 879–886. 2014. https://doi.org/10.1007/s13593-014-0209-6

SALA, F. C.; DA COSTA, C. P. Retrospectiva e tendência da alfacicultura Brasileira. Horticultura Brasileira, v. 30, p.187–194. 2012. https://doi.org/10.1590/S0102-05362012000200002

SÁNCHEZ, R. A.; SUNELL, L.; LABAVITCH, J. M.; BONNER, B. A. Changes in the endosperm cell walls of two Datura species before radicle protrusion. Plant Physiology, v. 93, p. 89–97.1990. https://doi.org/10.1104/pp.93.1.89

SEO, M. et al. Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism. Plant Journal, v. 48, p. 354–366. 2006. https://doi.org/10.1111/j.1365-313X.2006.02881.x

SEVENGOR, S.; YASAR, F.; KUSVURAN, S.; ELLIALTIOGLU, S. The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling. African Journal of Agricultural Research, v. 6, p. 4920–4924. 2011.

SINGH, D.; BASU, C.; MEINHARDT-WOLLWEBER, M.; ROTH, B. LEDs for energy efficient greenhouse lighting. Renewable and Sustainable Energy Reviews, v. 49, p. 139-147. 2015. https://doi.org/10.1016/j.rser.2015.04.117

SHARMA, P.; JHA, A. B.; DUBEY, R. S.; PESSARAKLI, M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, v. 2012, p. 1–26. 2012. https://doi.org/10.1155/2012/217037

SHEN, Y. et al. Red and blue light affect the formation of adventitious roots of tea cuttings (Camellia sinensis) by regulating hormone synthesis and signal transduction pathways of mature leaves. Frontiers Plant Science, v. 13, p. 943662. 2022. https://doi.org/10.3389/fpls.2022.943662

SHIMIZU, H. et al. Light environment optimization for lettuce growth in plant factory. In: 18th International Federation of Automatic Control (IFAC) World Congress. IFAC Proceedings Volumes, v. 44, p. 605–609. 2011.

SOLANO, C. J. et al. A LED-based smart experimental chamber to promote germination and growth of pea and melon plants: effect on the antioxidative metabolism. In: JOAO, S. D. (ed) Prime Archives in Agricultural Research. India: Hyderabad, 2021. p. 62-83.

TAIZ, L.; ZEIGER, E.; MALLER, IM.; MURPHY, A. Plant Physiology and Development. 6th ed. England: Oxford University Press, 2017.

TYLER, L. et al. Della proteins and gibberellin-regulated seed germination and floral development in Arabidopsis. Plant Physiology, v. 135, p. 1008–1019. 2004. https://doi.org/10.1104/pp.104.

039578

VELEZ-RAMIREZ, A. I.; VAN IEPEREN, W.; VREUGDENHIL, D.; MILLENAAR, F. F. Plants under continuous light. Trends Plant Science, v. 16, p. 310-318. 2011. https://doi.org/10.1016/j.tplants.2011.02.003

YANG, Y.; PEI, Q. Efficient blue?green and white light-emitting electrochemical cells based on poly[9,9-bis (3,6-dioxaheptyl)-fluorene-2,7-diyl]. Journal of Applied Physics, v. 81, p. 3294–3296. 1997. https://doi.org/10.1063/1.364313

YORIO, N. C. et al. Improving spinach, radish, and lettuce growth under red light-emitting diodes (LEDs) with blue light supplementation. HortScience, v. 36, p. 380–383. 2001. https://doi.org/10.21273/hortsci.36.2.380

ZHA, L. et al. Regulation of ascorbate accumulation and metabolism in lettuce by the red:blue ratio of continuous light using LEDs. Frontiers in Plant Science, v. 11, p. 704. 2020. https://doi.org/10.3389/fpls.2020.00704