Biocontrole de isolados de Streptomyces de solos áridos de Puerto Eten contra espécies de Fusarium e Cercospora

Jorge Luis Leiva Piedra; Walter Manuel Hoyos Alayo; Zaida Brenilda Chávez Romero; Jose Guillermo Gonzalez Cabeza; Miguel Angel Solano Cornejo

doi:10.17765/2176-9168.2026v19.e14157

Autores

Leiva-Piedra Jorge Luis Universidad Tecnológica del Perí https://orcid.org/0000-0002-2545-9438
Hoyos-Alayo Manuel Universidad Tecnológica del Perú https://orcid.org/0000-0002-8683-1635
Chávez-Romero Zaida Brenilda Universidad Tecnológica del Perú https://orcid.org/0000-0003-2802-3274
Gonzalez-Cabeza Jose Guillermo Universidad Privada Antenor Orrego https://orcid.org/0000-0003-3022-9423
Solano-Cornejo Miguel Angel Universidad Nacional Pedro Ruiz Gallo https://orcid.org/0000-0002-8221-7443

DOI:

https://doi.org/10.17765/2176-9168.2026v19.e14157

Palavras-chave:

Puerto Eten barren soils, Paired confrontation assay, Antifungal metabolites, Hydrolytic enzymes, 16S rRNA identification

Resumo

This study aimed to determine the biocontrol potential of Streptomyces isolates obtained from Puerto Eten barren soils against Fusarium spp. and Cercospora spp. Potential Streptomyces isolates from barren soils in Lambayeque were selected using actinomycete-oriented cultural, morphological and Gram-staining criteria, and were subsequently characterised by macroscopic, microscopic and 16S rRNA-based analyses. The isolates were evaluated through paired confrontation assays against Fusarium spp. and Cercospora spp., followed by extraction of crude bioactive compounds and comparison with a benzimidazole-based commercial fungicide in well diffusion assays. In the paired confrontation assay, relative inhibition ranged from 62.22% to 72.14% for Fusarium spp. and from 65.31% to 70.17% for Cercospora spp. In the well diffusion assay, crude extracts produced relative inhibition values of 66.64% to 70.00% for Fusarium spp. and 65.31% to 70.17% for Cercospora spp., while the fungicide control produced the highest inhibition. The isolates JCH7 and JCH3 showed positive lipase, protease, chitinase and cellulase activity, whereas JCH5 lacked chitinase activity. The 16S rRNA results related JCH7 and JCH3 to Streptomyces mutabilis, but their similarity values support a close relationship rather than a definitive species-level assignment. The JCH5 sequence showed an inconclusive low similarity and requires resequencing. Overall, the three isolates showed in vitro biocontrol potential against both fungal genera, with the strongest evidence based on inhibition and enzymatic activity.

Biografia do Autor

Leiva-Piedra Jorge Luis, Universidad Tecnológica del Perí

Engenheiro agrônomo com mestrado em proteção de culturas, especialista em controle biológico, atualmente professor pesquisador da Universidade Tecnológica do Peru.

Hoyos-Alayo Manuel, Universidad Tecnológica del Perú

Engenheiro químico com mestrado em Engenharia Ambiental e mais de 10 anos de experiência profissional e 3 em ensino universitário; com conhecimentos em: (1) Tratamento de águas e efluentes, (2) gestão de orçamentos e planos de trabalho, (3) conhecimento e uso de plataformas virtuais acadêmicas para gestão de pesquisa (por exemplo, gerenciadores bibliográficos, software estatístico, Vos Viewer, Bibliometrix, etc.).

Chávez-Romero Zaida Brenilda, Universidad Tecnológica del Perú

Engenheiro Industrial formado pela Universidade Católica Santo Toribio de Mogrovejo de Chiclayo e Ph.D em Fluidodinâmica e Processos de Engenharia Ambiental pela Universidade de Gênova, na Itália. Atualmente é professor da Escola de Engenharia Industrial da Universidade Tecnológica do Peru.

Gonzalez-Cabeza Jose Guillermo, Universidad Privada Antenor Orrego

Biólogo, formado pela Universidade Nacional de Trujillo; Mestre em Microbiologia Industrial e Biotecnologia (UNT); Mestre em Biotecnologia (Universidade Autônoma de Barcelona); Doutor em Biotecnologia (Universidade Autônoma de Barcelona) e com Estudos Avançados em Microbiologia (Universidade Autônoma de Barcelona). Atualmente, é professor das disciplinas de Microbiologia e Imunologia, Microbiologia e Parasitologia, Microbiologia Médica das Faculdades de Ciências da Saúde e Medicina da Universidade Privada Antenor Orrego de Trujillo (UPAO).

Solano-Cornejo Miguel Angel, Universidad Nacional Pedro Ruiz Gallo

Engenheiro em Indústrias Alimentares, formado pela Universidade Nacional Agrária La Molina. Mestre em Engenharia Ambiental e Segurança Industrial pela Universidade Nacional de Piura. Candidato a Mestre em Ciências em Tecnologia de Alimentos pela Universidade Nacional de Trujillo. Doutor em Engenharia de Alimentos pela Universidade Nacional de Santa. Professor na Escola Profissional de Indústrias Alimentares da Universidade Nacional Pedro Ruiz Gallo

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