Biocontrole de isolados de Streptomyces de solos áridos de Puerto Eten contra espécies de Fusarium e Cercospora

Jorge Luis Leiva-Piedra; Walter Manuel Hoyos-Alayo; Zaida Brenilda Chávez- Romero; Jose Guillermo Gonzalez-Cabeza; Miguel Angel Solano-Cornejo

doi:10.17765/2176-9168.2026v19.e14157

Authors

Leiva-Piedra Jorge Luis Universidad Tecnológica del Perú https://orcid.org/0000-0002-2545-9438
Hoyos-Alayo Manuel Universidad Tecnológica del Perú https://orcid.org/0000-0002-8683-1635
Chávez-Romero Zaida Brenilda Universidad Tecnológica del Perú https://orcid.org/0000-0003-2802-3274
Gonzalez-Cabeza Jose Guillermo Universidad Privada Antenor Orrego https://orcid.org/0000-0003-3022-9423
Solano-Cornejo Miguel Angel Universidad Nacional Pedro Ruiz Gallo https://orcid.org/0000-0002-8221-7443

DOI:

https://doi.org/10.17765/2176-9168.2026v19.e14157

Keywords:

Solos áridos de Puerto Eten, Ensaio de confronto pareado, Metabólitos antifúngicos, Enzimas hidrolíticas, Identificação por 16S rRNA

Abstract

Este estudo teve como objetivo determinar o potencial de biocontrole de isolados de Streptomyces obtidos de solos áridos de Puerto Eten contra Fusarium spp. e Cercospora spp. Os isolados potenciais de Streptomyces provenientes de solos áridos de Lambayeque foram selecionados por critérios culturais, morfológicos e coloração de Gram orientados para actinomicetos, e posteriormente caracterizados por análises macroscópicas, microscópicas e baseadas no gene 16S rRNA. Os isolados foram avaliados por ensaios de confronto pareado contra Fusarium spp. e Cercospora spp., seguidos da extração de compostos bioativos brutos e comparação com um fungicida comercial à base de benzimidazol por ensaio de difusão em poço. No confronto pareado, a inibição relativa variou de 62,22% a 72,14% para Fusarium spp. e de 65,31% a 70,17% para Cercospora spp. No ensaio de difusão em poço, os extratos brutos produziram inibição relativa de 66,64% a 70,00% para Fusarium spp. e de 65,31% a 70,17% para Cercospora spp., enquanto o controle fungicida apresentou a maior inibição. Os isolados JCH7 e JCH3 apresentaram atividade positiva de lipase, protease, quitinase e celulase, enquanto JCH5 não apresentou atividade de quitinase. Os resultados de 16S rRNA relacionaram JCH7 e JCH3 a Streptomyces mutabilis, mas os valores de similaridade sustentam uma relação próxima e não uma atribuição definitiva em nível de espécie. A sequência de JCH5 apresentou baixa similaridade inconclusiva e requer novo sequenciamento. Em conjunto, os três isolados demonstraram potencial de biocontrole in vitro contra ambos os gêneros fúngicos, com maior sustentação nos resultados de inibição e atividade enzimática.

Author Biographies

Leiva-Piedra Jorge Luis, Universidad Tecnológica del Perú

Agricultural engineer with a master's degree in crop protection, specialist in biological control, currently a research lecturer at the Technological University of Peru.

Hoyos-Alayo Manuel, Universidad Tecnológica del Perú

Chemical Engineer with a master’s degree in Environmental Engineering, over ten years of professional experience and three years in university teaching, with expertise in: (1) water and effluent treatment, (2) budget management and work planning, and (3) knowledge and use of academic virtual platforms for research management (e.g., reference managers, statistical software, VOSviewer, Bibliometrix, etc.).

Chávez-Romero Zaida Brenilda, Universidad Tecnológica del Perú

Industrial Engineer with a degree from the Catholic University of Santo Toribio de Mogrovejo in Chiclayo and a PhD in Fluid Dynamics and Environmental Engineering Processes from the University of Genoa, Italy. Currently a lecturer at the School of Industrial Engineering at the Technological University of Peru.

Gonzalez-Cabeza Jose Guillermo, Universidad Privada Antenor Orrego

Biologist, graduate of the National University of Trujillo; M.Sc. in Industrial Microbiology and Biotechnology (UNT); M.Sc. in Biotechnology (Autonomous University of Barcelona); Doctorate in Biotechnology (Autonomous University of Barcelona), and Advanced Studies in Microbiology (Autonomous University of Barcelona). He currently teaches Microbiology and Immunology, Microbiology and Parasitology, and Medical Microbiology at the Faculties of Health Sciences and Medicine at the Antenor Orrego Private University of Trujillo (UPAO).

Solano-Cornejo Miguel Angel, Universidad Nacional Pedro Ruiz Gallo

Food Industry Engineer, graduated from the National Agrarian University of La Molina. Master's Degree in Environmental Engineering and Industrial Safety from the National University of Piura. Candidate for a Master of Science in Food Technology from the National University of Trujillo. Doctorate in Food Engineering from the National University of Santa. Lecturer at the Professional School of Food Industries at the National University Pedro Ruiz Gallo.

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