Improvement of in vivo Mass Production Technique of Baculovírus spodoptera
Abstract
The in vivo multiplication of baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) in the specific host Spodoptera frugiperda features the issue of cannibalism of the pest species, with increase in costs and reduction of the final production. Current paper aims at improving the in vivo production method of SfMNPV based on the density of caterpillars and in the period of virus multiplication. Four-day old caterpillars of S. frugiperda were inoculated by virus suspension of isolate 6 (1 x 108 OB.mL-1) of SfMNPV in artificial diet. Caterpillars were kept at 31ºC in two densities (50 and 100 caterpillars/tray) and five periods for virus multiplication (48, 72, 96, 120 and 144 hours). After each period, the caterpillars of the two densities were frozen, weighed and ground to quantify occlusion bodies and the calculation of production parameters. Based on evaluated parameters, densities 50 and 100 caterpillars/tray and 96-h period are adequate for the improvement of in vivo production of isolate 6 of SfMNPV in caterpillars of S. frugiperda.References
CHAPMAN, J. W.; WILLIAMS, T.; ESCRIBANO, A.; CABALLERO, P.; CAVE, R. D.; GOULSON, D. Fitness consequences of cannibalism in the fall armyworm, Spodoptera frugiperda. Behavioral Ecology, v. 10, n. 3, p. 298-303, 1999.
ELVIRA, S.; GORRÍA, N.; MUÑOZ, D.; WILLIAMS, T.; CABALLERO, P. A simplified low-cost diet for rearing Spodoptera exigua (Lepidoptera: Noctuidae) and its affect on S. exigua nucleopolyhedrovirus production. Biological and Microbial Control, v. 103, n. 1, p. 17-24, 2010a.
ELVIRA, S.; WILLIAMS, T.; CABALLERO, P. Juvenile hormone analog technology: effects on larval cannibalism and the production of Spodoptera exigua (Lepidoptera: Noctuidae) nucleopolyhedrovirus. Journal of economic entomology, v. 103, n. 3, p. 577-582, 2010b.
FEDERICI, B. A. Naturally occurring baculoviruses for insect pest control. In: HALL, F. R.; MENN, J. J. (ed.). Methods in biotechnology: biopesticides, use and delivery. Totowa: Humana Presse, 1999. p. 301-320.
HASHIMOTO, Y.; HAYASHI, K.; HAYAKAWA, T.; UENO, Y.; SHIMOJO, E. I.; KONDO, A.; MIYASONO, M.; SANO, Y.; MATSUMOTO, T.; GRANADOS, R. R. Physical map of a Plutellaxylostella granulovirus genome. Applied Entomology and Zoology, v. 35, n. 1, p. 45-51, 2000.
LACEY, L. A.; GRZYWACZ, D.; SHAPIRO-ILAN, D. I.; FRUTOS, R.; BROWNBRIDGE, M.; GOETTEL, M. S. Insect pathogens as biological control agents: back to the future. Journal of invertebrate pathology, v. 132, p. 1-41, 2015.
LACEY, L. A. (ed.). Microbial Control of Insect and Mite Pests: From Theory to Practice. Academic Press, 2016. 444p.
MINISTÉRIO DA AGRICULTURA, PECUÁRIA E ABASTECIMENTO - MAPA. Instrução Normativa Conjunta, n° 1, de 6 de fevereiro de 2015. Diário Oficial da União, Brasília, Seção 1, 2015. Disponível em: http://www.in.gov.br/autenticidade.html. Acesso em: 20 jun. 2016.
NALIM, D. M. Biologia, nutrição quantitativa e controle de qualidade de populações de Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) em duas dietas artificiais. 1991. 174f. Tese (Doutorado em Ciências, área de concentração: entomologia), Universidade de São Paulo, Piracicaba, 1991.
RAMÍREZ-ARIAS, F. G.; LASA, R.; MURILLO, R.; NAVARRO-DE-LA-FUENTE, L.; MERCADO, G.; WILLIAMS, T. Post-mortem incubation influences occlusion body production in nucleopolyhedrovirus-infected larvae of Spodoptera frugiperda. Biological control, v. 135, p. 33-40, 2019.
RIOS-VELASCO, C.; GALLEGOS-MORALES, G.; BERLANGA-REYES, D.; CAMBERO-CAMPOS, J.; ROMO-CHACÓN, A. Mortality and production of occlusion bodies in Spodoptera frugiperda larvae (Lepidoptera: Noctuidae) treated with nucleopolyhedrovirus. Florida Entomologist, v. 95, n. 3, p. 752-757, 2012.
SILVA, C. S. B. DA; PARRA, J. R. P. New method rearing Spodoptera frugiperda in laboratory shows that larval cannibalism is not obligatory. Revista Brasileira de Entomologia, v. 57, n. 3, p. 347-349, 2013.
SLACK, J. M.; ARIF, B. M. The baculoviruses occlusion-derived virus: Virion structure and function. Advances in Virus Research, v. 69, p. 99-165, 2007.
SUBRAMANIAN, S.; SANTHARAM, G.; SATHIAH, N.; KENNEDY, J. S.; RABINDRA, R. J. Influence of incubation temperature on productivity and quality of Spodoptera litura nucleopolyhedrovirus. Biological Control, v. 37, n. 3, p. 367-374, 2006.
VALICENTE, F. H.; TUELHER, E. S.; PENA, R. C.; ANDREAZZA, R.; GUIMARÃES, M. R. F. Cannibalism and virus production in Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) larvae fed with two leaf substrates inoculated with Baculovirus spodoptera. Neotropical Entomology, v. 42, n. 2, p. 191-199, 2013.
VALICENTE, F. H.; TULHER, E. S.; BARROS, E. C. Processo de produção comercial de baculovírus em grande escala. Sete Lagoas: EMBRAPA-CNPMS, 2010a. 6p.
VALICENTE, F. H.; TUELHER, E. S.; BARROS, E. C. Processo de formulação do Baculovirus spodoptera em pó molhável. Sete Lagoas: EMBRAPA-CNPMS, 2010b. 5p. (Circular Técnica, 156).
VIEIRA, C. M.; TUELHER, E. S.; VALICENTE, F. H.; WOLFF, J. L. C. Characterization of a Spodoptera frugiperda multiple nucleopolyhedrovirus isolate that does not liquefy the integument of infected larvae. Journal of Invertebrate Pathology, v. 111, n. 2, p. 189-192, 2012.
ZAMORA-AVILÉS, N.; MARTÍNEZ, A. M.; PINEDA, S.; BRAVO-PITINÕ, A.; FIGUEROA, I.; LASA, R. Cool-textured diets for use in baculovirus production. Biocontrol Science and Technology, v. 27, n. 11, p. 1327-1338, 2017.
Os direitos autorais pertencem exclusivamente aos autores. Os direitos de licenciamento utilizado pelo periódico é a licença Creative Commons Attribution
Creative Commons Atribuição 4.0 Internacional. São permitidos o compartilhamento (cópia e distribuição do material em qualquer meio ou formato) e adaptação (remixar, transformar, e criar a partir do trabalho, mesmo para fins comerciais), desde que lhe atribuam o devido crédito pela criação original.