MICROBIOLOGICAL EVALUATION IN DIFFERENT CULTURE MEDIA AFTER THE REMOVAL OF HAAS EXPANDER APPLIANCE

  • Jéssica Ettienny Esteves Gomes UniCesumar
  • Renata Tereza Pinat da Silva UniCesumar
  • Rosely Suguino UniCesumar
  • Maria Paula Jacobucci Botelho UniCesumar
Keywords: Bacteria, Biofilm, Expansion

Abstract

Different types of microorganisms were analyzed after the removal of Haas expander appliance in children in the mixed denture phase. Twelve patients in the mixed denture phase, aged 6 -10 years old, from the Integrated Children Clinic of Cesumar and from an orthodontist clinic in Maringá PR Brazil, were treated with Haas expander appliance. After the appliance´s removal by swabbing, the bacterial plaque adherent to the acrylic surface was collected from the regions near the expanding screw (region B) and on the wider surface of the palate (region A). Biofilm was seeded in three petri plates, each with a different culture medium, namely, Mitis Salivarius agar, Sabouraud agar and Rogosa agar, to verify the presence of and count Streptococcus, fungi and Lactobacillus, respectively. Results showed predominance of fungi and lactobacilli in region B, whereas streptococci were prevalent in the acrylic region (region A). Increase in colony number of certain bacteria and fungi suggested periodic prophylaxis for plaque control in patients with Haas expander appliance.

Author Biographies

Jéssica Ettienny Esteves Gomes, UniCesumar
Acadêmica do Curso de Odontologia da UNICESUMAR - Centro Universitário de Maringá, Maringá, PR; Bolsista do Programa de Bolsas de Iniciação Científica da Unicesumar (PROBIC)
Renata Tereza Pinat da Silva, UniCesumar
Pesquisadora colaboradora, acadêmica do Curso de Odontologia da UNICESUMAR - Centro Universitário de Maringá, Maringá – PR
Rosely Suguino, UniCesumar
Orientadora, Docente da UNICESUMAR - Centro Universitário de Maringá, Maringá, PR
Maria Paula Jacobucci Botelho, UniCesumar
Coorientadora, Docente da UNICESUMAR - Centro Universitário de Maringá, Maringá, PR
Published
2013-07-16
Section
Artigos Originais