Análise in vitro da proliferação de células de melanoma submetidas à altas doses de vitamina D  : In vitro analysis of melanoma cell proliferation subjected to high doses of vitamin D

Bruno Henrique Moraes; Gabriela Ruva Fagundes; Paulo Roberto Costa de Almeida; Mário Cezar Saffi Junior; Felipe de Lara Janz; Giovani Marino Favero

doi:10.17765/2176-9206.2025v18e12972

Authors

Bruno Henrique Moraes State University of Ponta Grossa https://orcid.org/0009-0005-1587-5808
Gabriela Ruva Fagundes State University of Ponta Grossa https://orcid.org/0009-0008-4129-1162
Paulo Roberto Costa de Almeida State University of Ponta Grossa https://orcid.org/0000-0002-5719-4382
Mário Cezar Saffi Junior State University of Ponta Grossa https://orcid.org/0000-0002-4703-641X
Felipe de Lara Janz State University of Ponta Grossa https://orcid.org/0000-0002-6107-4375
Doutor State University of Ponta Grossa https://orcid.org/0000-0002-1946-3262

DOI:

https://doi.org/10.17765/2176-9206.2025v18e12972

Keywords:

Melanoma, Raios Ultravioleta, Vitamina D

Abstract

Skin cancer is one of the most common types of cancer worldwide, with rising incidence rates attributed to various risk factors, including ultraviolet radiation exposure. Vitamin D is a fat-soluble compound essential for maintaining bone health, supporting the immune system, and promoting cell growth. Vitamin D has also demonstrated anticancer properties due to its ability to inhibit neoplastic processes. This study aimed to investigate the effects of vitamin D on the proliferation and apoptosis of melanoma cells. For this purpose, the B16F10 cell line was treated with different concentrations of vitamin D for 13 hours. Subsequently, cell viability was analyzed using the MTT assay. The concentration 10 times higher than the physiological level showed the most pronounced cytotoxic effect, followed by the concentrations of 20 and 1 times. Vitamin D overload led to a decrease in the viability of B16F10 cells..

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Author Biographies

Bruno Henrique Moraes, State University of Ponta Grossa

Master's student in the Pharmaceutical Sciences Program at the State University of Ponta Grossa, Paraná

Gabriela Ruva Fagundes, State University of Ponta Grossa

Graduated in Pharmacy from the State University of Ponta Grossa (UEPG). Master's student in Pharmaceutical Sciences at UEPG. Experience in the areas of Tumor Immunology, Physiology, Pathophysiology and Cell Culture.

Paulo Roberto Costa de Almeida, State University of Ponta Grossa

Veterinarian. Postgraduate in Small Animal Medical Clinic. Postgraduate in Veterinary Cardiology. He has an advanced course in electrocardiography at the Heart Institute (InCor) of the Hospital das Clínicas of the Faculty of Medicine of São Paulo (USP). Master's student in Pharmaceutical Sciences with an emphasis on Cardiology.

Mário Cezar Saffi Junior, State University of Ponta Grossa

Dr. Mário Saffi has a degree in Medicine from Universidade Severino Sombra, a medical residency in Gynecology and Obstetrics from Conjunto Hospitalar do Mandaqui, a specialist title in Gynecology and Obstetrics (TEGO), a postgraduate degree in ultrasound in gynecology and obstetrics, specialization in pathology of the lower genital tract, specialization in bioethics from the University of Porto, Master's degree in cervical cancer screening from Nove de Julho University and PhD in perinatal palliative care from the State University of Ponta Grossa (UEPG). At the moment, he works as a gynecologist and obstetrician in his office in the city of Maringá-PR and is studying a Post-Doctorate linked to the Post-Graduate Program in Health Sciences, at the State University of Ponta Grossa. He works with research related to perinatal palliative care, scientometrics in the health area, gynecology and obstetrics.

Felipe de Lara Janz, State University of Ponta Grossa

Graduated in Pharmaceutical Sciences with Qualification in Clinical and Toxicological Analysis from the State University of Ponta Grossa - PR (2001-2005). Pedagogical Training - Degree in Biological Sciences (2021). Specialist in Teaching in Higher Education (2017). PhD in Medical Sciences with emphasis on Cell Growth, Hemodynamic and Hemostasis Disorders from the Faculty of Medicine of the University of São Paulo (2006-2010). International Scientific Internship at the University Hospital of the University of Salamanca (USAL, Spain, 2009). He has experience in cell culture working in the isolation, characterization, differentiation and cryopreservation of mesenchymal stem cells from different sources, especially amniotic fluid and human bone marrow. Post-doctorate in History of Science at the Faculty of Philosophy, Letters and Human Sciences of the University of São Paulo (2017).

Doutor, State University of Ponta Grossa

Current Head of the Department of General Biology at UEPG. Member of the Paraná Science and Technology Council. Member of the Special Commission for the Evaluation of Public Higher Education of Paraná CEA. Associate Professor at the Department of General Biology. from UEPG. He was President of the Paraná Council of Deans of Research and Postgraduate Studies (2022-2024). He was Pro-Rector of Research and Postgraduate Studies at the State University of Ponta Grossa (2018-2024). He has a degree in Pharmacy-Biochemistry from the State University of Ponta Grossa (2001), a master's degree in Science (Experimental Pathophysiology) from the University of São Paulo (2004) and a PhD in Allergy and Immunopathology from the University of São Paulo (2007). He is currently an Associate Professor (Livre Docente) at the State University of Ponta Grossa. He was Head of the General Biology Department (2010-2014), President of the Ethics Committee on the Use of Animals (2011-2014), member of the Human Ethics Committee and Chief Editor of the Publicatio UEPG Biological and Health Sciences Magazine (2008-2014 ). He is an evaluator of projects financed by CNPq, FINEP and Fundação Araucária. He has experience in the area of Cell Biology, Histology, Biochemistry with an emphasis on Human Anatomy and Pathophysiology, working mainly on the following topics: immuno-cellular interaction in pathophysiological processes, experimental oncology and nanoparticles. Presenter of the weekly column "Novidades da Ciência" on CBN radio in Ponta Grossa. He is one of the organizers and writers of the Nanoita column, on Science and Technology, in the Jornal Diário dos Campos. He has a partnership in technological development and technology transfer with Prati-Donaduzzi Indústria Farmacêutica and Startup Protein. He participates in Startup Academic Solutions. He has filed two invention patents.

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