Extração assistida por ultrassom de compostos do subproduto do Palmito Pupunha (Bainha mediana)

Isabella Carolina  Zampar; Gabriella Giovana Zampar; Natália Stevanato; Beatriz Cervejeira Bolanho  Barros; Camila da Silva

doi:10.17765/2176-9168.2023v16n2e10953

Isabella Carolina Zampar Universidade Estadual de Maringá – UEM https://orcid.org/0000-0003-2681-8823
Gabriella Giovana Zampar Universidade Estadual de Maringá – UEM
Natália Stevanato Universidade Estadual de Maringá – UEM https://orcid.org/0000-0001-5536-9524
Beatriz Cervejeira Bolanho Barros Universidade Estadual de Maringá – UEM, Departamento de Ciências Agronômicas, Campus de Umuarama https://orcid.org/0000-0003-1191-4740
Camila da Silva Universidade Estadual de Maringá - UEM https://orcid.org/0000-0002-7989-7046

DOI: https://doi.org/10.17765/2176-9168.2023v16n2e10953

Keywords: Antioxidant activity, Cavitation, Phenolic compounds, Soluble sugars

Abstract

This study aimed to evaluate the effect of ultrasound-assisted extraction (UAE) in obtaining soluble sugars (SS) from the peach palm by-product and to characterize the extract obtained in terms of phenol composition and antioxidant potential. The effect of the sample/solvent ratio on the extraction of AS was evaluated, then a Box-Behnken experimental design was applied to evaluate the effect of the independent variables (pH, temperature and time). Conventional extraction in an orbital shaker (EOS) was conducted for comparative purposes. The aqueous extracts were also characterized in terms of phenolic content and composition and antioxidant activity. The solubility of sugars in water was higher in the sample/water ratio of 2.5 mg mL-1. Based on the responses of the experimental design adopted, it was found that SA was obtained at pH = 4.0, temperature of 35 ºC and extraction time of 5 minutes, which resulted in the removal of 16.44 g 100 g-1 of AT. Furthermore, the UAE method was more effective in removing SS (15.23 g 100 g-1) and total phenolic compounds (308.51 mg 100 g-1) compared to the orbital agitation extraction method (EOS). However, in relation to antioxidant activity, the ABTS+ FRAP methods did not show a significant difference between UAE and EOS. It was possible to detect five phenolic acids presents in the middle sheath of the peach palm heart, whose contents were similar in the extractions performed by UAE and EOS.

Author Biographies

Isabella Carolina Zampar, Universidade Estadual de Maringá – UEM

Mestrado em Ciências Agrárias pela Universidade Estadual de Maringá (UEM), departamento de Ciências Agronômicas, câmpus regional de Umuarama (PR), Brasil.

Gabriella Giovana Zampar, Universidade Estadual de Maringá – UEM

Mestrado em Ciências Agrárias pela Universidade Estadual de Maringá (UEM), Departamento de Ciências Agronômicas, câmpus regional de Umuarama (PR), Brasil.

Natália Stevanato, Universidade Estadual de Maringá – UEM

Mestrado em Bioenergia pela Universidade Estadual de Maringá (UEM), departamento de engenharia química, Maringá (PR), Brasil.

Beatriz Cervejeira Bolanho Barros, Universidade Estadual de Maringá – UEM, Departamento de Ciências Agronômicas, Campus de Umuarama

Doutorado em Ciência e Tecnologia em Alimentos pela Universidade Estadual de Londrina (UEL). Professor Adjunto da Universidade Estadual de Maringá, Câmpus regional de Umuarama (PR), Brasil.

Camila da Silva, Universidade Estadual de Maringá - UEM

Doutorado em Engenharia Química pela Universidade Estadual de Maringá (UEM). Professor Adjunto da Universidade Estadual de Maringá, câmpus regional de Umuarama (PR), Brasil.

References

ALBERO, B.; TADEO, J. L.; PÉREZ, R. A. Ultrasound-assisted extraction of organic contaminants. Trends in Analytical Chemistry, v. 118, n. 12, p. 739-750, 2019. DOI: https://doi.org/10.1016/j.trac.2019.07.007.

AMOAH, J.; OGURA, K.; SCHMETZ, Q.; KONDO, A.; OGINO, C. Co-fermentation of xylose and glucose from ionic liquid pretreated sugar cane bagasse for bioethanol production using engineered xylose assimilating yeast. Biomass and Bioenergy, v. 128, n. 105283, 2019. DOI: https://doi.org/10.1016/j.biombioe.2019.105283.

BENZIE, I. F. F.; STRAIN, J. J. The Ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: The FRAP assay. Analytical Biochemistry, v. 239, n. 1, p. 70-79, 1996. DOI: https://doi.org/10.1006/abio.1996.0292.

BOLANHO, B. C.; DANESI, E. D. G.; BELÉIA, A. P. Characterization of flours made from peach palm (Bactris gasipaes Kunth) by-products as a new food ingredient. Journal of Food and Nutrition Research, v. 53, n. 1, p. 51-59, 2014.

CHAROENSIDDHI, S.; LORBEER, A. J.; LAHNSTEIN, J.; BULONE, V.; FRANCO, C. M. M.; ZHANG, W. Enzyme-assisted extraction of carbohydrates from the brown alga Ecklonia radiata: Effect of enzyme type, pH and buffer on sugar yield and molecular weight profiles. Process Biochemistry, v. 5, n. 10, p. 1503-1510, 2016. DOI: https://doi.org/10.1016/j.procbio.2016.07.014.

CHEMAT, F.; ZILL-E-HUMA.; KHAN, M. K. Applications of ultrasound in food technology: Processing, preservation and extraction. Ultrasonics Sonochemistry, v. 18, n. 4, p. 813-835, 2011. DOI: https://doi.org/10.1016/j.ultsonch.2010.11.023.

COSTA FILHO, D. V.; SILVA, A. J.; SILVA, P. A. P.; SOUSA, F. C. Aproveitamento de resíduos agroindustriais na elaboração de subprodutos. In: CONGRESSO INTERNACIONAL DAS CIÊNCIAS AGRÁRIAS, 2., 2017, João Pessoa. Anais [...]. João Pessoa: Instituto Internacional Despertando Vocações, 2017.

DONADONE, D. B. S.; GIOMBELLI, C.; SILVA, D. L. G.; STEVANATO, N.; SILVA, C.; BARROS, B. C. B. Ultrasound-assisted extraction of phenolic compounds and soluble sugars from the stem portion of peach palm. Journal of Food Processing and Preservation, v. 44, n. 5, p. 1-11, 2020. DOI: https://doi.org/10.1111/jfpp.14636.

GIOMBELLI, C.; IWASSA, I. J.; SILVA, C.; BARROS, B. C. B. Valorization of peach palm by-product through subcritical water extraction of soluble sugars and phenolic compounds. The Journal of Supercritical Fluids, v. 165, 104985, 2020. DOI: https://doi.org/10.1016/j.supflu.2020.104985.

GRAEFE, S.; DUFOUR, D.; ZONNEVELD, M.; RODRIGUEZ, F.; GONZALEZ, A. Peach palm (Bactris gasipaes) in tropical Latin America: implications for biodiversity conservation, natural resource management and human nutrition. Biodiversity and Conservation, v. 22, n. 1, p. 269-300, 2013. DOI: https://doi.org/10.1007/s10531-012-0402-3.

IWASSA, I. W.; RIBEIRO, M. A. S.; MEURER, E. C.; CARDOZO-FILHO, L.; BOLANHO, B. C.; SILVA, C. Effect of subcritical water processing on the extraction of compounds, composition, and functional properties of asparagus by-product. Journal of Food Process Engineering, v. 42, n. 1, e13060, 2019. DOI: https://doi.org/10.1111/jfpe.13060.

KHAN, M. K.; ABERT-VIAN, M.; FABIANO-TIXER, A. S.; DANGLES, O.; CHEMAT, F. Ultrasound-assisted extraction of polyphenols (flavanone glycosides) from orange (Citrus sinensis L.) peel. Food Chemistry, v. 119, n. 2, p. 851-858, 2010. DOI: https://doi.org/10.1016/j.foodchem.2009.08.046.

KUILA, A.; SINGH, A.; MUKHOPADHYAY, M.; BANERJEE, R. Process optimization for aqueous extraction of reducing sugar from cashew apple bagasse: A potential, lowcost substrate. LWT - Food Science and Technology, v. 44, n. 1, p. 62-66, 2011. DOI: https://doi.org/10.1016/j.lwt.2010.06.005.

KUMAR, K.; SRIVASTAV, S.; SHARANAGAT, V. S. Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products: A review. Ultrasonics Sonochemistry, v. 10, 105325, 2021. DOI: https://doi.org/10.1016/j.ultsonch.2020.105325.

LEVY, R. B.; CLARO, R. M.; BANDONI, D. H.; MONDINI, L.; MONTEIRO, C. A. Disponibilidade de “açúcares de adição” no Brasil: distribuição, fontes alimentares e tendência temporal. Revista Brasileira de Epidemiologia, v. 15, n. 1, p. 3-12, 2012. DOI: https://doi.org/10.1590/S1415-790X2012000100001.

MACHADO, M. T. C.; EÇA, K. S.; VIEIRA, G. S.; MENEGALLI, F. C.; MARTÍNEZ, J.; HUBINGER, M. D. Prebiotic oligosaccharides from artichoke industrial waste: evaluation of different extraction methods. Industrial Crops and Products, v. 76, p. 141-148, 2015. DOI: https://doi.org/10.1016/j.indcrop.2015.06.047.

NOWACKA, M.; WEDZIK, M. Effect of ultrasound treatment on microstructure, colour and carotenoid content in fresh and dried carrot tissue. Applied Acoustics, v. 103, p. 163-171, 2016. DOI: https://doi.org/10.1016/j.apacoust.2015.06.011.

OLIVEIRA, L. S.; PEREIRA, L. G. R.; AZEVEDO, J. A. G.; PEDREIRA, M. S.; LOURDES, D. R. S.; BOMFIM, M. A. D.; BARREIROS, D. C.; BRITO, R. L. L. Caracterização nutricional de silagens do coproduto da pupunha. Revista Brasileira de Saúde e Produção Animal, v. 11, n. 2, p. 426-439, 2010.

OMS - Organização Mundial da Saúde. Diretriz: ingestão de açúcar para adultos e crianças. 2015.

PANESAR, R.; KAUR, S.; PANESAR, P. S. Production of microbial pigment sutilizing agro-industrial waste: a review. Current Opinion in Food Science, v. 1, n. 5, p. 70-76, 2015. DOI: https://doi.org/10.1016/j.cofs.2014.12.002.

PARK, J. W.; PARK, K. G.; LEE, N. Y.; LEE, J. H.; LEE, J. W. Enhanced extraction of reducing sugars from fruit of Hoveni adulcis with treatment of cellulase and sequential production of ethanol and acetic acid containing ampelopsin from extracted reducing sugars. Industrial Crops and Products, v. 139, 111522, 2019. DOI: https://doi.org/10.1016/j.indcrop.2019.111522.

PENTEADO JUNIOR, J. F.; SANTOS, A. F.; NEVES, E. J. M. Aspectos do agronegócio do palmito de pupunha no Brasil. Colombo: Embrapa Florestas, 2014. (Documentos, 275).

PLAZA, M.; MARINA, M. L. Pressurized hot water extraction of bioactives. Trends in Analytical Chemistry, v. 116, p. 236-247, 2019. DOI: https://doi.org/10.1016/j.trac.2019.03.024.

POLACHINI, T. C.; MULET, A.; TELIS-ROMERO, J.; CÁRCEL, J. A. Influence of high-intensity ultrasound application on the kinetics of sugar release from acid suspensions of artichoke (Cynaras colymus) biomass. Chemical Engineering and Processing - Process Intensification, v. 45, 107681, 2019. DOI: https://doi.org/10.1016/j.cep.2019.107681.

QUINTAS, M.; BRANDÃO, T. R. S.; SILVA, C. L. M. Modelling autocatalytic behavior of a food model system - Sucrose thermal degradation at high concentrations. Journal of Food Engineering, v. 78, n. 2, p. 537-545, 2007. DOI: https://doi.org/10.1016/j.jfoodeng.2005.10.031.

RAYO-MENDEZ, L. M.; GÓMEZ, A. V.; TADINI, C. C. T. Extraction of soluble sugars from banana puree to obtain a matrix rich in non-starch polysaccharides. Food Chemistry, v. 249, p. 539-546, 2019. DOI: https://doi.org/10.1016/j.foodchem.2019.05.079.

REZENDE, Y. R.; NOGUEIRA, J. P.; NARAIN, N. Comparison and optimization of conventional and ultrasound-assisted extraction for bioactive compounds and antioxidant activity from agro-industrial acerola (Malpighiae marginata DC) residue. LWT - Food Science and Technology, v. 85, p. 158-169, 2017. DOI: https://doi.org/10.1016/j.lwt.2017.07.020.

SCHMIDT, P.; ROSSI JUNIOR, P.; TOLEDO, L. M.; NUSSIO, L. G.; ALBUQUERQUE, D. S.; MEDURI, B. Perdas fermentativas e composição bromatológica da entre casca do palmito pupunha ensilada com aditivos químicos. Revista Brasileira de Zootecnia, v. 39, n. 2, p. 262-267, 2010. DOI: http://dx.doi.org/10.1590/S1516-35982010000200005.

SHAHIDI, F.; ZHONG, Y. Measurement of antioxidant activity. Journal of Functional Foods, v. 18, p. 757-781, 2015. DOI: https://doi.org/10.1016/j.jff.2015.01.047.

SHIRSATH, S. R.; SONAWANE, S. H.; GOGATE, P. R. Intensification of extraction of natural products using ultrasonic irradiations - a review of current status. Chemical Engineering and Processing: Process Intensification, v. 53, p. 10-23, 2012. DOI: https://doi.org/10.1016/j.cep.2012.01.003.

THAIPONG, K.; BOONPRAKOB, U.; CROSBY, K.; CISNEROS-ZEVALLOS, L.; BYRNE, D. H. Comparison of ABTS, DPPH, FRAP and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis, v. 19, p. 669-675, 2006. DOI: 10.1016/j.jfca.2006.01.003.

WANG, J.; WANG, J.; VANGA, S. K.; RAGHAVAN, V. High-intensity ultrasound processing of kiwifruit juice: Effects on the microstructure, pectin, carbohydrates and rheological properties. Food Chemistry, v. 313, 126121, 2020. DOI: https://doi.org/10.1016/j.foodchem.2019.126121.

WEN, C.; ZHANG, J.; ZHANG, H.; DUAN, Y.; MA, H. Effects of divergent ultrasound pretreatment on the structure of watermelon seed protein and the antioxidant activity of its hydrolysates. Food Chemistry, v. 99, 125165, 2019. DOI: https://doi.org/10.1016/j.foodchem.2019.125165.

WEN, C.; ZHANG, J.; ZHANG, H.; DZAH, C. S.; ZANDILE, M.; DUAN, Y.; MA, H.; LUO, X. Advances in ultrasound assisted extraction of bioactive compounds from cash crops - A review. Ultrasonics Sonochemistry, v. 48, p. 538-549, 2018. DOI: https://doi.org/10.1016/j.ultsonch.2018.07.018.

YAO, Y.; PAN, Y.; LIU, S. Power ultrasound and its applications: A state-of-the-art review. Sonochemistry Ultrasonics, v. 62, 104722, 2020. DOI: https://doi.org/10.1016/j.ultsonch.2019.104722.

ZHAO, Y.; WEN, C.; FENG, Y.; ZHANG, J.; HE, Y.; DUAN, Y.; ZHANG, H.; MA, H. Effects of ultrasound-assisted extraction on the structural, functional and antioxidant properties of Dolichos lablab L. Protein. Process Biochemistry, v. 101, p. 274-284, 2021. DOI: https://doi.org/10.1016/j.procbio.2020.11.027.

ZHU, Z.; GUAN, Q.; KOUBAA, M.; BARBA, F. J.; ROOHINEJAD, S.; CRAVOTTO, G.; YANG, X.; LI, S.; HE, J. HPLC-DAD-ESI-MS2 analytical profile of extracts obtained from purples weet potato after green ultrasound assisted extraction. Food Chemistry, v. 21, p. 391-400, 2017. DOI: https://doi.org/10.1016/j.foodchem.2016.07.157.

Ultrasound-assisted extraction of compounds from the peach palm heart BY-product (Median sheath)

Abstract

Author Biographies

References