MAXIMIZING SECOND GENERATION ETHANOL FROM BIOMASS
DOI:
https://doi.org/10.17765/2176-9168.2024v17n.Especial.e12577%20Keywords:
Sugarcane Bagasse, Physical Pre-treatment, Microwave, Ultrasound, BiofuelAbstract
In recent years, there has been a growing national interest in fuel ethanol production due to its renewable nature. The conversion of biomass into ethanol has emerged as a globally researched and promising alternative, particularly in the fields of electricity generation and liquid biofuel production. Second-generation ethanol, derived from lignocellulosic biomass, is obtained through advanced chemical processes or biotechnology with the aim of breaking down cellulose into fermentable sugars. This study aimed to investigate pre-treatments using ultrasound and microwaves to hydrolyze sugarcane bagasse. Samples of sugarcane bagasse were washed, dried, suspended in acidic solution (1 and 0.1 M) or distilled water, and subjected to ultrasound and microwave treatment for varying exposure times ranging from 10 to 30 minutes. The materials were then filtered, washed, and fermented until reaching 1 °Brix. Distillation was performed after fermentation using a simple distiller. Prior to fermentation, samples were analyzed for reducing and total reducing sugars, as well as hydroxymethylfurfural, while alcohol content was measured post-fermentation. Treatments were conducted in four repetitions. The results showed that microwave pre-treatments with water or 0.1M acidic solution produced musts with higher sugar content, resulting in more alcoholic distillates. However, ultrasound was ineffective in sugar release, rendering alcoholic fermentation unfeasible. These findings underscore the critical role of selecting the appropriate pre-treatment method for achieving efficient second-generation ethanol production from lignocellulosic biomass.References
ADEWUYI, Y. G. Sonochemistry: environmental science and engineering applications. Industrial & Engineering Chemistry Research, v. 40, n. 22, p. 4681-4715, 2001. https://doi.org/10.1021/ie010096l
AFEDZI, A. E. K.; Parakulsuksatid, P. Recent advances in process modifications of simultaneous saccharification and fermentation (SSF) of lignocellulosic biomass for bioethanol production. Biocatalysis and Agricultural Biotechnology, p. 102961, 2023. https://doi.org/10.1016/j.bcab.2023.102961
ARAÚJO, D. F. C.; SOBRINHO, F. L. A. O futuro dos biocombustíveis: Análise do cenário atual e perspectivas para o setor no Brasil. Geopauta, v. 7, p. e12766-e12766, 2023. https://doi.org/10.22481/rg.v7.e2023.e12766
BADIEI, M.; ASIM, N.; JAHIM, J. M.; SOPIAN, K. Comparison of Chemical Pretreatment Methods for Cellulosic Biomass. APCBEE Procedia, v. 9, p. 170-174, 2014. https://doi.org/10.1016/j.apcbee.2014.01.030
BAIG, K. S.; WU, J.; TURCOTTE, G. Future prospects of delignification pretreatments for the lignocellulosic materials to produce second generation bioethanol. International Journal of Energy Research, v. 43, n. 4, p. 1411-1427, 2019. https://doi.org/10.1002/er.4292
BALAT, M.; BALAT, H.; ÖZ, C. Progress in bioethanol processing. Progress in Energy and Combustion Science, v. 34, n. 5, p. 551-573, 2008. https://doi.org/10.1016/j.pecs.2007.11.001
BINOD, P.; SATYANAGALAKSHMI, K.; SINDHU, R.; JANU, K. U.; SUKUMARAN, R. K.; PANDEY, A. Short duration microwave assisted pretreatment enhances the enzymatic saccharification and fermentable sugar yield from sugarcane bagasse. Renewable Energy, v. 36, n. 2, p. 466-470, 2010. https://doi.org/10.1016/j.renene.2011.06.007
BIZERRA, A. M. C.; QUEIROZ, J. L. A. DE; COUTINHO, D. A. M. O impacto ambiental dos combustíveis fósseis e dos biocombustíveis: as concepções de estudantes do ensino médio sobre o tema. Revista Brasileira de Educação Ambiental, v. 13, n. 3, p. 299-315, 2018.
CAMARGOS, C. H. M.; SILVA, R. A. P.; CSORDAS, Y.; SILVA, L. L.; REZENDE, C. A. Experimentally designed corn biomass fractionation to obtain lignin nanoparticles and fermentable sugars, Industrial Crops and Products, v. 140, p. 111649, 2019. https://doi.org.10.1016/j.indcrop.2019.111649
DUQUE, A.; ÁLVAREZ, C.; DOMÉNECH, P.; MANZANARES, P.; MORENO, A. D. Advanced bioethanol production: From novel raw materials to integrated biorefineries. Processes, v. 9, n. 2, p. 206, 2021. https://doi.org/10.3390/pr9020206
FAZZI, L. R.; SIMÕES, A. F.; ALMEIDA, P. S.; OLIVEIRA, B. D. A regulação de biocombustíveis no Brasil e nos EUA no contexto da mitigação das mudanças climáticas e do correlato Acordo de Paris. Revista Gestão & Sustentabilidade Ambiental, v. 9, p. 104-119, 2020. https://doi.org/10.19177/rgsa.v9e02020104-119
FERREIRA, J. Etanol de segunda geração: definição e perspectivas. Revista Conexão Eletrônica, v. 12, p. 11, 2015.
GALBE, M.; ZACCHI, G. Pretreatment of Lignocellulosic Materials for Efficient Bioethanol Production. In: OLSSON, L. (Ed.). Biofuels. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. v. 108, p. 41-65. https://doi.org/10.1007/10_2007_070
GOGATE, P. R.; PANDIT, A. B. A review of imperative technologies for wastewater treatment I: oxidation technologies at ambient conditions. Advances in Environmental Research, v. 8, n. 3-4, p. 501-551, 2014. https://doi.org/10.1016/S1093-0191(03)00032-7
INSTITUTO ADOLFO LUTZ. Normas Analíticas do Instituto Adolfo Lutz. Métodos físico-químicos para análises de alimentos. 4ª ed. (1ª Edição digital), 2008. 1020 p.
KUMAR, P.; BARRETT, D. M.; DELWICHE, M. J.; STROEVE, P. Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production. Industrial & Engineering Chemistry Research, v. 48, n. 8, 2011. https://doi.org/10.1021/ie801542g
KUSTER MORO, M.; SPOSINA, R.; SILVA, A. S.; FUJIMOTO, M. D.; BON, E.P.S; MELO JR., P. A.; Secchi, A. R. Pré-tratamento da biomassa de cana-de-açúcar por extrusão com dupla-rosca. In: Simpósio Nacional de Bioprocessos e Simpósio de Hidrólise Enzimática de Biomassa. Anais..., 5 set. 2015.
LARA-SERRANO, M.; SA?EZ ANGULO, F.; NEGRO, M. J.; MORALES-DELA ROSA, S.; CAMPOS-MARTIN, J. M.; FIERRO, J. L. Second-generation bioethanol production combining simultaneous fermentation and saccharification of IL-pretreated barley straw. ACS Sustainable Chemistry & Engineering, v. 6, n. 5, p. 7086-7095, 2018. https://doi.org/10.1021/acssuschemeng.8b00953
LEI, H.; CYBULSKA, I.; JULSON, J. Hydrothermal Pretreatment of Lignocellulosic Biomass and Kinetics. Journal of Sustainable Bioenergy Systems, v. 3, n. 4, p. 250-259, 2013. http://dx.doi.org/10.4236/jsbs.2013.34034
MATSAKAS, L.; RAGHAVENDRAN, V.; YAKIMENKO, O.; PERSSON, G.; OLSSON, E.; ROVA, U.; CHRISTAKOPOULOS, P. Lignin-first biomass fractionation using a hybrid organosolv–steam explosion pretreatmet technology improves the saccharification and fermentability of spruce biomass. Bioresource technology, v. 273, p. 521-528, 2019. https://doi.org/10.1016/j.biortech.2018.11.055
MOHAPATRA, S.; MISHRA, C.; BEHERA, S. S.; THATOI, H. Application of pretreatment, fermentation and molecular techniques for enhancing bioethanol production from grass biomass – A review. Renewable and Sustainable Energy Reviews, v. 78, p. 1007-1032, 2017. https://doi.org/10.1016/j.rser.2017.05.026
NELSON, N. A photometric adaptation of Somogyi method for determination of glucose. Journal of Biologic Chemistry, v. 153, n. 2, p. 375-380, 1960. https://doi.org/10.1016/S0021-9258(18)71980-7
OLIVEIRA, L. R. M. Estudo de alternativas de pré-tratamento e hidrólise do bagaço e palha de cana-de-açúcar para obtenção de etanol a partir de celulose, Tese de Doutorado, Universidade de São Paulo, 123p., 2012.
PRADHAN, D.; TSEGAYE, B.; MATHEW, S.; JAISWAL, S.; JAISWAL, A. Ultrasound-Assisted Pretreatment of Lignocellulosic Biomass for Bioethanol Production, pp.365-387, 2023. In: Konur, O. (ed.) Bioethanol Fuel Production Processes, 1 ed., CRC Press: Boca Raton, 2023. https://doi.org/10.1201/9781003226536-24
SANSEVERINO, A. M. Microondas em síntese orgânica. Quimica Nova, v. 25, n. 4, p. 660–667, 2002. https://doi.org/10.1590/S0100-40422002000400022
SATARI, B.; KARIMI, K.; KUMAR, R. Cellulose solvent-based pretreatment for enhanced second-generation biofuel production: a review. Sustainable energy & fuels, v. 3, n. 1, p. 11-62, 2019. https://doi.org/10.1039/C8SE00287H
TAYYAB, M.; NOMAN, A.; ISLAM, W.; WAHEED, S.; ARAFAT, Y.; ALI, F.; LIN, W. Bioethanol production from lignocellulosic biomass by environment-friendly pretreatment methods: a review. Applied Ecology & Environmental Research, v. 16, n. 1, 2018. http://dx.doi.org/10.15666/aeer/1601_225249
WANG, X. FANG, G.; HU, C.; DU, T. Application of ultrasonic waves in activation of microcrystalline cellulose. Journal of applied polymer science, v. 109, n. 5, p. 2762-2767, 2008. https://doi.org/10.1002/app.27975
ZHANG, S.; KESHWANI, D. R.; XU, Y.; HANNA, M. A. Alkali combined extrusion pretreatment of corn stover to enhance enzyme saccharification, Industrial Crops and Products, v. 37, p. 352-357, 2012. https://doi.org/10.1016/J.INDCROP.2011.12.001
ZHANG, Y.; CAI, D.; YONG, Q.; YU, S. Microwave-assisted acid pretreatment of alkali lignin: Effect on characteristics and pyrolysis behavior. Bioresource Technology, v. 256, p. 90-96, 2018. https://doi.org/10.1016/j.biortech.2017.12.022
ZHAO, X.; CHENG, K.; LIU, D. Organosolv pretreatment of lignocellulosic biomass for enzymatic hydrolysis. Applied Microbiology and Biotechnology, v. 101, n. 20, p. 8277-8294, 2017. https://doi.org/10.1007/s00253-009-1883-1
ZLOTORZYNSKI, A. The Application of Microwave Radiation to Analytical and Environmental Chemistry. Critical Reviews in Analytical Chemistry, v. 25, n. 1, p. 43-76, 1995. https://doi.org/10.1080/10408349508050557
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Revista em Agronegócio e Meio Ambiente
This work is licensed under a Creative Commons Attribution 4.0 International License.
A Revista se reserva o direito de efetuar, nos originais, alterações de ordem normativa, ortográfica e gramatical, com o intuito de manter o padrão culto da língua, respeitando, porém, o estilo dos autores. As opiniões emitidas pelos autores são de sua exclusiva responsabilidade.Os direitos autorais pertencem exclusivamente aos autores. Os direitos de licenciamento utilizado pelo periódico é a licença Creative Commons Attribution
Creative Commons Atribuição 4.0 Internacional. São permitidos o compartilhamento (cópia e distribuição do material em qualquer meio ou formato) e adaptação (remixar, transformar, e criar a partir do trabalho, mesmo para fins comerciais), desde que lhe atribuam o devido crédito pela criação original.
