Evaluation of positive NADPH-diaphorase myenteric neurons in diabetic rats supplemented with Acetyl-L-Carnitine

DOI: https://doi.org/10.17765/2176-9206.2021v14Supl.1.e8410
Keywords: Gastrointestinal tract, Myenteric plexus, Nitric oxide

Abstract

The objective was to evaluate supplementation with acetyl-L-carnitine (ALC) on myenteric neurons of the ileum of rats after induction of diabetes. Diabetic animals supplemented with ALC (DC), diabetic (D), normoglycemic animals supplemented with ALC (CC) and normoglycemic (C) were used. NADPH-d neurons were quantified and measured. There was a reduction in blood glucose and water intake in the DC group. The neuronal density in 12.72mm² of ileum was similar in the four groups (p>0.05): DC (558.8 ± 220.2), D (513.4 ± 72.01), CC (645.2 ± 144.9) and C (934 ± 248.5). The mean cell body area of neurons (μm²) in diabetic animals, DC (303.9 ± 114.2) and D (285.4 ± 111.8), were greater than in the normoglycemic groups, CC (173.6 ± 53.78) and C (158.4 ± 53.73). The ileum area (mm²) was larger in animals of the diabetic groups, CD (190.96) and D (171.62) compared to the normoglycemic groups: CC (138.04) and C (130.04). However, in the DC group, both areas were larger than in D (p<0.05). Thus, a slight increase in neuronal population can be inferred. The data indicated that ALC did not interfere with mechanisms that promote an increase in the production of nitric oxide (NO) by myenteric neurons of the ileum and that the greater dilation of the ileum in the DC group could be the result of a side effect of the dose of carnitine used.

Author Biographies

Angela Maria Pereira Alves, Universidade Estadual de Maringá - UEM
Doctor´s degree in Biological Sciences (Cell Biology) of the Universidade Estadual de Maringá (UEM). Adjunct professor at the Universidade Estadual de Maringá, Maringá PR Brazil.
Marli Aparecida Defani, Universidade Estadual de Maringá - UEM
Doctor´s degree in Biological Sciences (Cell Biology) of the Universidade Estadual de Maringá (UEM). Adjunct professor at the Universidade Estadual de Maringá, Maringá PR Brazil.
Éder Paulo Belato Alves, Universidade Estadual de Maringá - UEM
Doctor´s degree in Biological Sciences (Cell Biology) of the Universidade Estadual de Maringá (UEM). Adjunct professor at the Universidade Estadual de Maringá, Maringá PR Brazil.
Stephanie Carvalho Borges, Universidade Estadual de Maringá - UEM
Doctor´s degree in Biological Sciences (Cell and Molecular Biology) at the Universidade Estadual de Maringá (UEM), Maringá PR Brazil.
Cristina Elena Prado Teles Fregonesi, Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP
Doctor´s degree in Biological Sciences (Cell Biology) at the Universidade Estadual de Maringá (UEM). Assistant Professor of the Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Presidente Prudente SP Brazil.
Sandra Regina Stabille, Universidade Estadual de Maringá - UEM
Doctor´s degree in Morphofunctional Studies at the Universidade de São Paulo (USP/SP). Emeritus professor at the Universidade Estadual de Maringá, Maringá PR Brazil.
Marcilio Hubner de Miranda Neto, Universidade Estadual de Maringá - UEM
Doctor´s degree in Sciences at the Universidade de São Paulo (USP/SP). Full professor at the Universidade Estadual de Maringá, Maringá PR Brazil.

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Published
2021-12-20
Issue
Vol 14 No (Supl. 1) (2021): Supl. 1 - dez.
Section
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