PHYTOCHEMICAL ANALYSIS AND THE EFFECT OF ETHANOL EXTRACT FROM THE LEAVES OF Solanum grandiflorum RUIZ ON Candida albicans in vitro

  • Dryelle Vieira Rodrigues Faculdade São Lucas
  • Renato Abreu Lima Fundação Universidade Federal de Rondônia
Keywords: Candidiasis, Inhibition, Natural Resources, Secondary Metabolites.

Abstract

Current research identifies secondary metabolites from the ethanol extract of S. grandiflorum leaves and evaluates the in vitro fungus activity on C. albicans. Five mm diameter discs with fungus culture were placed in the center of petri plates with BDA medium with 4 filter paper symmetrical discs, each with 1 mL of vegetal extract. Positive control comprised discs with distilled water, whereas negative control consisted of discs with a chemical substance. Experimental design comprised three repetitions per treatment. Evaluation verified fungus growth every 24 hours by measurement of the colonies. Data underwent analysis of variance as means were compared by Tukey´s test at 5% significance. Test which verified secondary metabolites provided positive results, featuring alkaloids, cardiotonic glycosides, volatile cumarins, flavonoids, saponins, triterpenes and derived products of free anthracenes. Diogenin and Solasodin were evidenced by the chromatographic test. Extract from the S. grandiflorum leaves inhibited C. albicans. Results revealed the importance of employing natural resources against fungi and providing new perspectives in the use of antimicrobial substances.

Author Biographies

Dryelle Vieira Rodrigues, Faculdade São Lucas
Discente do curso de Ciências Biológicas Ciências Biológicas pela Faculdade São Lucas
Renato Abreu Lima, Fundação Universidade Federal de Rondônia
Doutorando em Biodiversidade e Biotecnologia Universidade Federal do Amazonas – UFAM
Published
2014-02-10
Section
Artigos Originais